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Full length protein corresponding to Cow Osteocalcin.
Database link: P02820
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.
Our Abpromise guarantee covers the use of ab13421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/1 - 1/10.|
|WB||Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 11 kDa.|
|IHC-Fr||Use a concentration of 1 - 10 µg/ml.|
|IHC-P||Use a concentration of 1 - 10 µg/ml.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 16820635|
ICC/IF image of ab13421 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12421, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM
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