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Full length protein (Cow).
Our Abpromise guarantee covers the use of ab13421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/1 - 1/10.|
|WB||Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 11 kDa.|
|IHC-Fr||Use a concentration of 1 - 10 µg/ml.|
|IHC-P||Use a concentration of 1 - 10 µg/ml.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 16820635|
ICC/IF image of ab13421 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12421, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM