Overview

  • Product nameAnti-Osteocrin antibody
    See all Osteocrin primary antibodies
  • Description
    Rabbit polyclonal to Osteocrin
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human Osteocrin.

    (Peptide available as ab40903.)

  • Positive control
    • This antibody gave a positive signal in Human bone lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab39649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).

Target

Anti-Osteocrin antibody images

  • Anti-Osteocrin antibody (ab39649) at 1 µg/ml + Human bone tumor tissue lysate - total protein (ab29359) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 15 kDa
    Observed band size : 15 kDa
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References for Anti-Osteocrin antibody (ab39649)

ab39649 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Ms Heart Ms Sk Musc Ms Liver U2OS)
Loading amount 20 µg
Specification Ms Heart Ms Sk Musc Ms Liver U2OS
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Aug 12 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"