For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Recombinant full length protein (Mouse) expressed in NSO cells.
Our Abpromise guarantee covers the use of ab11503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Blocking||Use at an assay dependent concentration. Blocks osteopontin in smooth muscle cell samples.|
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 65 kDa.|
|Neutralising||Use a concentration of 1 - 3 µg/ml. The Neutralization Dose50 (ND50) for anti-mouse osteopontin is approximately 1 to 3 µg/ml in the presence of 2 µg/ml of recombinant mouse osteopontin using 293 cells.|
|Indirect ELISA||Use a concentration of 0.5 - 1 µg/ml.|
|IHC-Fr||Use at an assay dependent dilution. PubMed: 17224478|