Anti-Osteopontin antibody (ab11503)
Key features and details
- Goat polyclonal to Osteopontin
- Suitable for: Indirect ELISA, ICC/IF, Blocking, IHC-Fr, WB, Neutralising
- Reacts with: Mouse, Rat
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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Overview
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Product name
Anti-Osteopontin antibody
See all Osteopontin primary antibodies -
Description
Goat polyclonal to Osteopontin -
Host species
Goat -
Tested applications
Suitable for: Indirect ELISA, ICC/IF, Blocking, IHC-Fr, WB, Neutralisingmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant full length protein (Mouse) expressed in NSO cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: PBS, 5% Trehalose -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Indirect ELISA |
Use at an assay dependent concentration.
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ICC/IF | (1) |
Use at an assay dependent concentration.
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Blocking |
Use at an assay dependent concentration.
Blocks osteopontin in smooth muscle cell samples. |
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IHC-Fr |
Use at an assay dependent concentration. PubMed: 17224478
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WB |
Use at an assay dependent concentration.
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Neutralising |
Use at an assay dependent concentration.
The Neutralization Dose50 (ND50) for anti-mouse osteopontin is approximately 1 to 3 µg/ml in the presence of 2 µg/ml of recombinant mouse osteopontin using 293 cells. |
Notes |
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Indirect ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Blocking
Use at an assay dependent concentration. Blocks osteopontin in smooth muscle cell samples. |
IHC-Fr
Use at an assay dependent concentration. PubMed: 17224478 |
WB
Use at an assay dependent concentration. |
Neutralising
Use at an assay dependent concentration. The Neutralization Dose50 (ND50) for anti-mouse osteopontin is approximately 1 to 3 µg/ml in the presence of 2 µg/ml of recombinant mouse osteopontin using 293 cells. |
Target
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Function
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. -
Tissue specificity
Bone. Found in plasma. -
Sequence similarities
Belongs to the osteopontin family. -
Post-translational
modificationsExtensively phosphorylated on clustered serine residues.
N- and O-glycosylated.
Phosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 20750 Mouse
- Entrez Gene: 25353 Rat
- SwissProt: P10923 Mouse
- SwissProt: P08721 Rat
- Unigene: 288474 Mouse
- Unigene: 8871 Rat
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Alternative names
- BNSP antibody
- Bone sialoprotein 1 antibody
- Bone sialoprotein I antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (13)
ab11503 has been referenced in 13 publications.
- Zhang S et al. Induction of ferroptosis promotes vascular smooth muscle cell phenotypic switching and aggravates neointimal hyperplasia in mice. Mol Med 28:121 (2022). PubMed: 36192693
- Liao H et al. Osteopontin-integrin signaling positively regulates neuroplasticity through enhancing neural autophagy in the peri-infarct area after ischemic stroke. Am J Transl Res 14:7726-7743 (2022). PubMed: 36505285
- Qian D et al. Hyperin up-regulates miR-7031-5P to promote osteogenic differentiation of MC3T3-E1 cells. Histol Histopathol N/A:18579 (2022). PubMed: 36633331
- Xin D et al. MSCs-extracellular vesicles attenuated neuroinflammation, synapse damage and microglial phagocytosis after hypoxia-ischemia injury by preventing osteopontin expression. Pharmacol Res 164:105322 (2021). PubMed: 33279596
- Giopanou I et al. Osteopontin drives KRAS-mutant lung adenocarcinoma. Carcinogenesis 41:1134-1144 (2020). PubMed: 31740923