Overview

  • Product name
  • Description
    Goat polyclonal to Osteopontin
  • Host species
    Goat
  • Tested applications
    Suitable for: Blocking, ICC/IF, WB, Neutralising, Indirect ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant full length protein (Mouse) expressed in NSO cells.

  • Positive control
    • Mouse osteopontin expressed in NSO cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab11503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Blocking Use at an assay dependent concentration. Blocks osteopontin in smooth muscle cell samples.
ICC/IF 1/50.
WB Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 65 kDa.
Neutralising Use a concentration of 1 - 3 µg/ml. The Neutralization Dose50 (ND50) for anti-mouse osteopontin is approximately 1 to 3 µg/ml in the presence of 2 µg/ml of recombinant mouse osteopontin using 293 cells.
Indirect ELISA Use a concentration of 0.5 - 1 µg/ml.
IHC-Fr Use at an assay dependent dilution. PubMed: 17224478

Target

  • Function
    Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
    Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
  • Tissue specificity
    Bone. Found in plasma.
  • Sequence similarities
    Belongs to the osteopontin family.
  • Post-translational
    modifications
    Extensively phosphorylated on clustered serine residues.
    N- and O-glycosylated.
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BNSP antibody
    • Bone sialoprotein 1 antibody
    • BSP I antibody
    • BSPI antibody
    • Early T lymphocyte activation 1 antibody
    • ETA 1 antibody
    • ETA1 antibody
    • MGC110940 antibody
    • Nephropontin antibody
    • OPN antibody
    • Osteopontin antibody
    • osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein antibody
    • OSTP_HUMAN antibody
    • PSEC0156 antibody
    • secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) antibody
    • Secreted phosphoprotein 1 antibody
    • SPP 1 antibody
    • SPP-1 antibody
    • SPP1 antibody
    • SPP1/CALPHA1 fusion antibody
    • Urinary stone protein antibody
    • Uropontin antibody
    see all

References

This product has been referenced in:
  • Sharon Y  et al. Tumor-derived osteopontin reprograms normal mammary fibroblasts to promote inflammation and tumor growth in breast cancer. Cancer Res 75:963-73 (2015). IHC-P ; Mouse . Read more (PubMed: 25600648) »
  • Shibasaki M  et al. Pitavastatin Reduces Inflammation in Atherosclerotic Plaques in Apolipoprotein E-Deficient Mice with Late Stage Renal Disease. PLoS One 10:e0138047 (2015). Read more (PubMed: 26367531) »

See all 7 Publications for this product

Customer reviews and Q&As

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse bone cells)
Specification
mouse bone cells
Fixative
Formaldehyde
Permeabilization
Yes - 0.5% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5%
Username

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Verified customer

Submitted Nov 24 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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