Overview

  • Product name
  • Description
    Rabbit polyclonal to Osteopontin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Mouse Osteopontin aa 230-280 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    ESQESADQSDV IDSQASSKAS LEHQSHKFHS HKDKLVLDPK SKEDDRYLKF


    Database link: P10923

  • Positive control
    • Mouse kidney lysates; Rat shin bone and hippocampus tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab216402 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Predicted molecular weight: 32 kDa.
IHC-P 1/100 - 1/500.

Target

  • Function
    Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
    Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
  • Tissue specificity
    Bone. Found in plasma.
  • Sequence similarities
    Belongs to the osteopontin family.
  • Post-translational
    modifications
    Extensively phosphorylated on clustered serine residues.
    N- and O-glycosylated.
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BNSP antibody
    • Bone sialoprotein 1 antibody
    • BSP I antibody
    • BSPI antibody
    • Early T lymphocyte activation 1 antibody
    • ETA 1 antibody
    • ETA1 antibody
    • MGC110940 antibody
    • Nephropontin antibody
    • OPN antibody
    • Osteopontin antibody
    • osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein antibody
    • OSTP_HUMAN antibody
    • PSEC0156 antibody
    • secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) antibody
    • Secreted phosphoprotein 1 antibody
    • SPP 1 antibody
    • SPP-1 antibody
    • SPP1 antibody
    • SPP1/CALPHA1 fusion antibody
    • Urinary stone protein antibody
    • Uropontin antibody
    see all

Images

  • Anti-Osteopontin antibody (ab216402) at 1/300 dilution + mouse kidney lysates

    Secondary
    Goat anti-rabbit IgG (H+L), HRP conjugated at 1/5000 dilution

    Predicted band size: 32 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat shin bone tissue tissue labeling Osteopontin with ab216402 at 1/300 dilution followed by conjugation to the secondary antibody and DAB staining.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat hippocampus tissue tissue labeling Osteopontin with ab216402 at 1/300 dilution followed by conjugation to the secondary antibody.

References

ab216402 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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