The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1.25 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
FunctionBinds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Tissue specificityBone. Found in plasma.
Sequence similaritiesBelongs to the osteopontin family.
Post-translational modificationsExtensively phosphorylated on clustered serine residues. N- and O-glycosylated. Phosphorylation sites are present in the extracelllular medium.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Osteopontin with ab33046 at 4µg/ml. Arrows indicate positively labelled epithelial cells of renal tubule. Magnification: 400X.
References for Anti-Osteopontin antibody (ab33046)
This product has been referenced in:
Paino F et al. Histone deacetylase inhibition with valproic acid downregulates osteocalcin gene expression in human dental pulp stem cells and osteoblasts: evidence for HDAC2 involvement. Stem Cells32:279-89 (2014).
Read more (PubMed: 24105979) »
Meng YH et al. Elevated expression of connective tissue growth factor, osteopontin and increased collagen content in human ascending thoracic aortic aneurysms. Vascular22:20-7 (2014).
Read more (PubMed: 23508392) »