• Product nameAnti-Osteopontin antibody
    See all Osteopontin primary antibodies
  • Description
    Rabbit polyclonal to Osteopontin
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Guinea pig, Cow
  • Immunogen

    A region within synthetic peptide: LSKVSREFHS HEFHSHEDML VVDPKSKEED KHLKFRISHE LDSASSEVN, corresponding to C terminal amino acids 252-300 of Human Osteopontin

  • Positive control
    • WB: Jurkat cell lysate. IHC-P: Human kidney.



Our Abpromise guarantee covers the use of ab33046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1.5625e+006.
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.


  • FunctionBinds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
    Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
  • Tissue specificityBone. Found in plasma.
  • Sequence similaritiesBelongs to the osteopontin family.
  • Post-translational
    Extensively phosphorylated on clustered serine residues.
    N- and O-glycosylated.
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BNSP antibody
    • Bone sialoprotein 1 antibody
    • BSP I antibody
    • BSPI antibody
    • Early T lymphocyte activation 1 antibody
    • ETA 1 antibody
    • ETA1 antibody
    • MGC110940 antibody
    • Nephropontin antibody
    • OPN antibody
    • Osteopontin antibody
    • osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein antibody
    • OSTP_HUMAN antibody
    • PSEC0156 antibody
    • secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) antibody
    • Secreted phosphoprotein 1 antibody
    • SPP 1 antibody
    • SPP-1 antibody
    • SPP1 antibody
    • SPP1/CALPHA1 fusion antibody
    • Urinary stone protein antibody
    • Uropontin antibody
    see all

Anti-Osteopontin antibody images

  • Anti-Osteopontin antibody (ab33046) at 1.25 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg

    HRP conjugated anti Rabbit IgG at 1/50000 dilution

    Predicted band size : 35 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Osteopontin with ab33046 at 4µg/ml. Arrows indicate positively labelled epithelial cells of renal tubule. Magnification: 400X.

References for Anti-Osteopontin antibody (ab33046)

This product has been referenced in:
  • Paino F  et al. Histone deacetylase inhibition with valproic acid downregulates osteocalcin gene expression in human dental pulp stem cells and osteoblasts: evidence for HDAC2 involvement. Stem Cells 32:279-89 (2014). ICC/IF . Read more (PubMed: 24105979) »
  • Meng YH  et al. Elevated expression of connective tissue growth factor, osteopontin and increased collagen content in human ascending thoracic aortic aneurysms. Vascular 22:20-7 (2014). IHC-P ; Human . Read more (PubMed: 23508392) »

See all 3 Publications for this product

Product Wall

Application Western blot
Sample Rat Tissue lysate - whole (Skin)
Loading amount 15 µg
Specification Skin
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5%

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Submitted Oct 31 2006