• Product nameAnti-Osteopontin antibody
    See all Osteopontin primary antibodies
  • Description
    Goat polyclonal to Osteopontin
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide:


    , corresponding to C terminal amino acids 288-299 of Human Osteopontin

  • Positive control
    • Human thymus, tonsil and spleen lysate. Jurkat and MOLT4 lysate.


Associated products


Our Abpromise guarantee covers the use of ab36125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 34 kDa).

Blocking peptide available ab45753.


  • FunctionBinds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
    Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
  • Tissue specificityBone. Found in plasma.
  • Sequence similaritiesBelongs to the osteopontin family.
  • Post-translational
    Extensively phosphorylated on clustered serine residues.
    N- and O-glycosylated.
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BNSP antibody
    • Bone sialoprotein 1 antibody
    • BSP I antibody
    • BSPI antibody
    • Early T lymphocyte activation 1 antibody
    • ETA 1 antibody
    • ETA1 antibody
    • MGC110940 antibody
    • Nephropontin antibody
    • OPN antibody
    • Osteopontin antibody
    • osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein antibody
    • OSTP_HUMAN antibody
    • PSEC0156 antibody
    • secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) antibody
    • Secreted phosphoprotein 1 antibody
    • SPP 1 antibody
    • SPP-1 antibody
    • SPP1 antibody
    • SPP1/CALPHA1 fusion antibody
    • Urinary stone protein antibody
    • Uropontin antibody
    see all

Anti-Osteopontin antibody images

  • Anti-Osteopontin antibody (ab36125) at 0.3 µg/ml + Human Thymus lysate (RIPA buffer, 30µg total protein per lane).

    Predicted band size : 34 kDa
  • Anti-Osteopontin antibody (ab36125) at 1 µg/ml + lysate prepared from MOLT4 cells (RIPA buffer) at 30 µg

    Predicted band size : 34 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • Ab36125 (1ug/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

    A) Staining in the germinal centre and interfollicular region.

    B) Positive cells in intraepithelial region.

References for Anti-Osteopontin antibody (ab36125)

This product has been referenced in:
  • Wang Y  et al. Peptide nanofibers preconditioned with stem cell secretome are renoprotective. J Am Soc Nephrol 22:704-17 (2011). WB ; Mouse . Read more (PubMed: 21415151) »
  • Chen KC  et al. Evaluation of subchronic toxicity of pet food contaminated with melamine and cyanuric acid in rats. Toxicol Pathol 37:959-68 (2009). Read more (PubMed: 19801503) »

See all 2 Publications for this product

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