Anti-Osteopontin antibody (ab53772)

Overview

  • Product nameAnti-Osteopontin antibody
    See all Osteopontin primary antibodies
  • Description
    Rabbit polyclonal to Osteopontin
  • SpecificityThis antibody detects endogenous levels of total osteopontin protein.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from human osteopontin

  • Positive control
    • Extracts from LOVO cells

Properties

Applications

Our Abpromise guarantee covers the use of ab53772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 35 kDa).

Target

  • FunctionBinds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
    Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
  • Tissue specificityBone. Found in plasma.
  • Sequence similaritiesBelongs to the osteopontin family.
  • Post-translational
    modifications
    Extensively phosphorylated on clustered serine residues.
    N- and O-glycosylated.
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BNSP antibody
    • Bone sialoprotein 1 antibody
    • BSP I antibody
    • BSPI antibody
    • Early T lymphocyte activation 1 antibody
    • ETA 1 antibody
    • ETA1 antibody
    • MGC110940 antibody
    • Nephropontin antibody
    • OPN antibody
    • Osteopontin antibody
    • osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein antibody
    • OSTP_HUMAN antibody
    • PSEC0156 antibody
    • secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) antibody
    • Secreted phosphoprotein 1 antibody
    • SPP 1 antibody
    • SPP-1 antibody
    • SPP1 antibody
    • SPP1/CALPHA1 fusion antibody
    • Urinary stone protein antibody
    • Uropontin antibody
    see all

Anti-Osteopontin antibody images

  • All lanes : Anti-Osteopontin antibody (ab53772) at 1/500 dilution

    Lane 1 : Extracts from LOVO cells
    Lane 2 : Extracts from LOVO cells, plus immunising peptide


    Predicted band size : 35 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

References for Anti-Osteopontin antibody (ab53772)

ab53772 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your patience.

I received the following additional information:

For ab53772: This antibody does not bind to the region aa130-170.

For ab72526:Internal data that indicate that the epitope does indeed fall wit...

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Thank you for contacting us.

I have contacted the lab regarding ab72526 and will let you know what I find out.

As for osteopontin antibodies binding to the region of residues 130 to 170:

ab8448 (http://www.abcam.com/Osteo...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"