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- Osteopontin antibody (ab8448)
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- Osteopontin antibody (ab8448)
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Immunocytochemistry/ Immunofluorescence - Osteopontin antibody (ab8448)
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Product Name
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Osteopontin antibody
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See all Osteopontin antibodies (14)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Osteopontin
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Immunogen
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Synthetic peptide: CKSKKFRRPDIQYPD, corresponding to amino acids 170-183 of Human Osteopontin.
(Peptide available as ab9078).
BLAST 'CKSKKFRRPDIQYPD' with  or 
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Reacts with
(species key)
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Hu, Rat, Dog, Pig
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Specificity
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This antibody recognises the full length osteopontin protein (which runs at 66 kD on westerns), as well as the C-terminal fragments of both thrombin and MMP-cleaved OPN. This antibody recognises the 32 kD MMP-cleaved C-fragment, but not the 40 kD N-terminal fragment.
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Tested applications
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ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
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Recommended dilutions
Western blot: at 1/1000 will strongly detect approximately 250 ng of OPN protein on a blot.
ICC/IF: 1/1000.
IHC-Fr: Suggested working dilution of 1/100 (see PMID: 16128620).
IHC-F: 1/500.
Immunohistochemistry: use at 1/100 - 1/500. No pretreatment is required.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Secreted
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Research areas
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Cancer >> Tumor biomarkers >> Other
Stem Cells >> Mesenchymal Stem Cells >> Osteogenesis
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
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Relevance
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Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine.
Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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serum with 0.02% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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85.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Osteopontin antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Osteopontin antibody images, references, products related to ab8448 and other tools.
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Osteopontin antibody images:
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 - Osteopontin antibody (ab8448)
The osteopontin antibody (ab8448) is used at 1:1000 dilution on a blot with 250ng human osteopontin (lane 2)and MMP-cleaved osteopontin (lane 3) Renu Agnihotri
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- Osteopontin antibody (ab8448)
OPN is cleaved by MMP to yield 2 fragments, which migrate at 40kD(N terminal) and 32kD (C terminal). The C terminal fragment can undergo further cleavage by both of these MMPs (see Agnihotri et al, JBC 2001 for further details). The epitope recognised by ab8448 is shown in violet. This antibody detects the full length OPN and the 32kD fragment. It does not recognise the 40kD fragment. Based on Agnihotri et al, JBC 2001
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Immunocytochemistry/ Immunofluorescence - Osteopontin antibody (ab8448)
ICC/IF image of ab8448 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8448, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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- Osteopontin antibody (ab8448)
Breast tumour section. Osteopontin is a normal component of elastic fibers in the breast (shown heavily stained in this section). There is also weak staining of the extracellular matrix. Osteopontin is not believed to be expressed inside breast tumour cells, and there is no staining in the intracellular region of the breast cells in this section.
Osteopontin antibody (ab8448) used at 1:100-1:300. No antigen retrieval is required.
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References for Osteopontin antibody (ab8448)
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This product has been used in: (two most recent references)
Song YR et al. Activation of hypoxia-inducible factor attenuates renal injury in rat remnant kidney. Nephrol Dial Transplant 25:77-85 (2010). IHC-P; Rat. PubMed: 19737871
Ricchiuti V et al. Estradiol increases angiotensin II type 1 receptor in hearts of ovariectomized rats. J Endocrinol 200:75-84 (2009). PubMed: 18931023
See all 15 publication references for this product.
If you publish research using ab8448 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Osteopontin
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Osteopontin antibody - more information
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Customer reviews (feedback) regarding Osteopontin antibody |
Customer FAQs regarding Osteopontin antibody |
Protocols for Osteopontin antibody |
Price and availability of products related to Osteopontin antibody |
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Osteopontin antibody - related products:
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Osteopontin antibody - other tools:
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Contact Abcam with a Technical Enquiry about Osteopontin antibody |
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