Overview

  • Product nameAnti-Osteopontin antibody
    See all Osteopontin primary antibodies
  • Description
    Rabbit polyclonal to Osteopontin
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51(RIAVICFCLL GITCAIPVKQ ADSGSSEEKQ LYNKYPDAVA TWLNPDPSQK) of Human Osteopontin (NP_000573).

  • Positive control
    • THP1 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab95090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionBinds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
    Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
  • Tissue specificityBone. Found in plasma.
  • Sequence similaritiesBelongs to the osteopontin family.
  • Post-translational
    modifications
    Extensively phosphorylated on clustered serine residues.
    N- and O-glycosylated.
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BNSP antibody
    • Bone sialoprotein 1 antibody
    • BSP I antibody
    • BSPI antibody
    • Early T lymphocyte activation 1 antibody
    • ETA 1 antibody
    • ETA1 antibody
    • MGC110940 antibody
    • Nephropontin antibody
    • OPN antibody
    • Osteopontin antibody
    • osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein antibody
    • OSTP_HUMAN antibody
    • PSEC0156 antibody
    • secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) antibody
    • Secreted phosphoprotein 1 antibody
    • SPP 1 antibody
    • SPP-1 antibody
    • SPP1 antibody
    • SPP1/CALPHA1 fusion antibody
    • Urinary stone protein antibody
    • Uropontin antibody
    see all

Anti-Osteopontin antibody images

  • Anti-Osteopontin antibody (ab95090) at 1 µg/ml (in 5% skim milk / PBS buffer) + THP1 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 35 kDa
  • Anti-Osteopontin antibody (ab95090) at 1 µg/ml + Osteopontin protein (Active) (ab81549) at 0.1 µg/ml

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 20 minutes

References for Anti-Osteopontin antibody (ab95090)

ab95090 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Goat Tissue lysate - whole (Skim Goat Milk)
Gel Running Conditions Reduced Denaturing (8-12% Bis Tris Polyacrylamide)
Loading amount 350 µg
Specification Skim Goat Milk
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10000µg/mL · Temperature: 27°C
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Submitted Sep 13 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"