Anti-Osteopontin antibody [EPR3688] (ab91655)


  • Product nameAnti-Osteopontin antibody [EPR3688]
    See all Osteopontin primary antibodies
  • Description
    Rabbit monoclonal [EPR3688] to Osteopontin
  • Tested applicationsSuitable for: ICC/IF, WB, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • WB: MOLT-4 or HeLa cell lysate. Ms heart and kidney. Weaker signal with Ms brain. IHC: Human colon adenocarcinoma. ICC/IF: HeLa cells.
  • General notes


    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab91655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 35 kDa.Can be blocked with Osteopontin peptide (ab189325).
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for IP.
  • Target

    • FunctionBinds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
      Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
    • Tissue specificityBone. Found in plasma.
    • Sequence similaritiesBelongs to the osteopontin family.
    • Post-translational
      Extensively phosphorylated on clustered serine residues.
      N- and O-glycosylated.
      Phosphorylation sites are present in the extracelllular medium.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • BNSP antibody
      • Bone sialoprotein 1 antibody
      • BSP I antibody
      • BSPI antibody
      • Early T lymphocyte activation 1 antibody
      • ETA 1 antibody
      • ETA1 antibody
      • MGC110940 antibody
      • Nephropontin antibody
      • OPN antibody
      • Osteopontin antibody
      • osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein antibody
      • OSTP_HUMAN antibody
      • PSEC0156 antibody
      • secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) antibody
      • Secreted phosphoprotein 1 antibody
      • SPP 1 antibody
      • SPP-1 antibody
      • SPP1 antibody
      • SPP1/CALPHA1 fusion antibody
      • Urinary stone protein antibody
      • Uropontin antibody
      see all

    Anti-Osteopontin antibody [EPR3688] images

    • Immunofluorescence staining of HT-29 cells with purified ab91655 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 100% methanol. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.

    • All lanes : Anti-Osteopontin antibody [EPR3688] (ab91655) at 1/1000 dilution

      Lane 1 : MOLT-4 cell lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size : 35 kDa

    References for Anti-Osteopontin antibody [EPR3688] (ab91655)

    This product has been referenced in:
    • Guo X  et al. Hypoxia promotes glioma-associated macrophage infiltration via periostin and subsequent M2 polarization by upregulating TGF-beta and M-CSFR. Oncotarget 7:80521-80542 (2016). IHC, ICC/IF ; Human . Read more (PubMed: 27602954) »
    • Ferreira R  et al. Comparative proteomic analyses of urine from rat urothelial carcinoma chemically induced by exposure to N-butyl-N-(4-hydroxybutyl)-nitrosamine. Mol Biosyst 11:1594-602 (2015). WB ; Rat . Read more (PubMed: 25739956) »

    See all 6 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (HEART VALVE)
    Antigen retrieval step Heat mediated
    Permeabilization No
    Specification HEART VALVE
    Blocking step endogenous peroxidases as blocking agent for 10 minute(s) · Concentration: 0.3% · Temperature: RT°C
    Fixative Formaldehyde

    Hessah Alshammari

    Verified customer

    Submitted Nov 15 2016

    Application Western blot
    Sample Human Cell lysate - whole cell (Hec1a)
    Gel Running Conditions Reduced Denaturing (10)
    Loading amount 45 µg
    Specification Hec1a
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

    Mrs. Monika Schumacher

    Verified customer

    Submitted Jul 22 2015

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Antigen retrieval step Other
    Sample Mouse Tissue sections (mouse knee)
    Specification mouse knee
    Permeabilization Yes - 0.2% Triton X-100 and PBS
    Fixative Formaldehyde

    Abcam user community

    Verified customer

    Submitted Mar 19 2014

    Thank you for contacting us.

    The sequence in one of the Q/A was added in error, the correct sequence is a 13 amino acid sequence form 270-300aa of human Osteopontin protein.

    I hope this information is helpful to you. Please do not hes...

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    I would actually recommend an alternative secondary antibody ab97051, which is part of our AbExcel series of secondary antibodies. The link for ab97051 is given below:

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    Thank you for contacting us.

    Please find attached the requested data.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Thank you for calling Abcam earlier today.

    I am sorry that the initial antibody that you purchased did not prove to be successful for you. As we discussed on the phone, I am sending you ab63856 as a free of charge replacement for this antibody...

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    I am sorry to hear that this lot of the antibody is not working as expected.

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    Thank you for your answer and confirming that your customer added the modifier. The modifier step is inherent to the protocol.
    I have to repeat that this kit can only be used for purified antibodies. The antibodies need to be purified before the c...

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    Thank you and your customer for taking the time to complete our questionnaire and contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from this antibody.
    The details your customer has kindly provided will...

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