The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa).
Use a concentration of 5 - 20 µg/ml.
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Bone. Found in plasma.
Belongs to the osteopontin family.
Extensively phosphorylated on clustered serine residues. N- and O-glycosylated. Phosphorylation sites are present in the extracelllular medium.
Ning S et al. Genetic differences in osteogenic differentiation potency in the thoracic ossification of the ligamentum flavum under cyclic mechanical stress. Int J Mol Med39:135-143 (2017).
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