Overview

  • Product name
    Anti-Osteopontin antibody - N-terminal
    See all Osteopontin primary antibodies
  • Description
    Rabbit polyclonal to Osteopontin - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Osteopontin (N terminal). (18 amino acid peptide; NP_001238759).
    Database link: P10451

  • Positive control
    • Human bladder tissue and lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab181440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa).
IHC-P Use a concentration of 5 - 20 µg/ml.

Target

  • Function
    Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
    Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
  • Tissue specificity
    Bone. Found in plasma.
  • Sequence similarities
    Belongs to the osteopontin family.
  • Post-translational
    modifications
    Extensively phosphorylated on clustered serine residues.
    N- and O-glycosylated.
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BNSP antibody
    • Bone sialoprotein 1 antibody
    • BSP I antibody
    • BSPI antibody
    • Early T lymphocyte activation 1 antibody
    • ETA 1 antibody
    • ETA1 antibody
    • MGC110940 antibody
    • Nephropontin antibody
    • OPN antibody
    • Osteopontin antibody
    • osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein antibody
    • OSTP_HUMAN antibody
    • PSEC0156 antibody
    • secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) antibody
    • Secreted phosphoprotein 1 antibody
    • SPP 1 antibody
    • SPP-1 antibody
    • SPP1 antibody
    • SPP1/CALPHA1 fusion antibody
    • Urinary stone protein antibody
    • Uropontin antibody
    see all

Images

  • Lane 1 : Anti-Osteopontin antibody - N-terminal (ab181440) at 1 µg/ml
    Lane 2 : Anti-Osteopontin antibody - N-terminal (ab181440) at 2 µg/ml

    All lanes : Human bladder tissue lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 36 kDa
    Observed band size: 37 kDa (why is the actual band size different from the predicted?)

  • Immunohistochemical analysis of paraffin-embedded Human bladder tissue labeling Osteopontin with ab181440 at 5 µg/ml.

  • Immunohistochemical analysis of paraffin-embedded Human bladder tissue labeling Osteopontin with ab181440 at 20 µg/ml.

References

This product has been referenced in:
  • Sampayo-Escobar V  et al. Osteopontin plays a pivotal role in increasing severity of respiratory syncytial virus infection. PLoS One 13:e0192709 (2018). Read more (PubMed: 29677209) »
  • Ning S  et al. Genetic differences in osteogenic differentiation potency in the thoracic ossification of the ligamentum flavum under cyclic mechanical stress. Int J Mol Med 39:135-143 (2017). Read more (PubMed: 28004120) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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