Overview

  • Product nameOsteopontin Mouse ELISA KitSee all Osteopontin kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 4 pg/ml
  • Range
    2.74 pg/ml - 2000 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 85.46 78% - 105%
    Serum 115.6 103% - 122%
    Plasma 110.4 87% - 130%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s Osteopontin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Osteopontin in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Osteopontin coated on a 96-well plate. Standards and samples are pipetted into the wells and Osteopontin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Osteopontin antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Osteopontin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • FunctionBinds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
    Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
  • Tissue specificityBone. Found in plasma.
  • Sequence similaritiesBelongs to the osteopontin family.
  • Post-translational
    modifications
    Extensively phosphorylated by FAM20C in the extracelllular medium at multiple sites within the S-x-E/pS motif.
    N- and O-glycosylated.
  • Cellular localizationSecreted.
  • Target information above from: UniProt accession P10451 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names
    • BNSP
    • Bone sialoprotein 1
    • Bone sialoprotein I
    • BSPI
    • Early T lymphocyte activation 1
    • ETA 1
    • MGC110940
    • Nephropontin
    • OPN
    • Osteopontin
    • OSTP_HUMAN
    • Secreted phosphoprotein 1
    • Secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1)
    • Secreted phosphoprotein 1 (osteopontin bone sialoprotein)
    • SPP-1
    • SPP1
    • Urinary stone protein
    • Uropontin
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

Osteopontin Mouse ELISA Kit images

  • Representative standard curve using ab100734

Protocols

References for Osteopontin Mouse ELISA Kit (ab100734)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abreview for Osteopontin Mouse ELISA Kit

Good Good 4/5 (Ease of Use)
Abreviews

Sample: Mouse (Lavage fluid)
Specification: Lavage fluid
Amount of Sample: Dilutions of pooled lavage µg
Type of Kit Used: ELISA
Positive control: recombinant Osteopontin spiked in diluent
Did you use the Abcam Kit protocol?: Yes
Username

Abcam user community

Verified customer

Submitted Mar 18 2013

Thank youfor your inquiry.

The quote for ab8448 has been send in a separate email.

I am happy to confirm that we offer ELISA kits for Osteopontin:

ab100618 (Osteopontin Human ELISA Kit)

http://www.abcam.com/index...

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Thank you for contacting us.

This kit has not been optimised for use with tissue lysates but should be suitable for that purpose.

For the sample preparation, please note that there should not be more than 0.1% SDS present in the bu...

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Thank you for contacting us.  This kit has not been optimized for use with tissue lysates but should be suitable for that purpose.  A RIPA buffer should be fine as long as it meets the lysis buffer specifications below.  Please note that there should n...

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Abreview for Osteopontin Mouse ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Mouse Tissue sections (Lung homogenate)
Specification: Lung homogenate
Amount of Sample: 10 µg
Type of Kit Used: ELISA
Positive control: Recombinant mouse OPN of known concentration.
Did you use the Abcam Kit protocol?: Yes
Username

Abcam user community

Verified customer

Submitted May 13 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"