Overview

  • Product nameAnti-Osteoprotegerin antibody [EPR3592]
    See all Osteoprotegerin primary antibodies
  • Description
    Rabbit monoclonal [EPR3592] to Osteoprotegerin
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt,IP or WB
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human TNFRSF11B.

  • Positive control
    • Fetal kidney lysate. Human kidney tissue. Human heart tissue. HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab124820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt,IP or WB.
  • Target

    • FunctionActs as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local RANKL/OPG ratio. May also play a role in preventing arterial calcification. May act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis.
    • Tissue specificityHighly expressed in adult lung, heart, kidney, liver, spleen, thymus, prostate, ovary, small intestine, thyroid, lymph node, trachea, adrenal gland, testis, and bone marrow. Detected at very low levels in brain, placenta and skeletal muscle. Highly expressed in fetal kidney, liver and lung.
    • Involvement in diseaseDefects in TNFRSF11B are the cause of juvenile Paget disease (JPD) [MIM:239000]; also known as hyperostosis corticalis deformans juvenilis or hereditary hyperphosphatasia or chronic congenital idiopathic hyperphosphatasia. JPD is a rare autosomal recessive osteopathy that presents in infancy or early childhood. The disorder is characterized by rapidly remodeling woven bone, osteopenia, debilitating fractures, and deformities due to a markedly accelerated rate of bone remodeling throughout the skeleton. Approximately 40 cases of JPD have been reported worldwide. Unless it is treated with drugs that block osteoclast-mediated skeletal resorption, the disease can be fatal.
    • Sequence similaritiesContains 2 death domains.
      Contains 4 TNFR-Cys repeats.
    • Post-translational
      modifications
      N-glycosylated. Contains sialic acid residues.
      The N-terminus is blocked.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • MGC29565 antibody
      • OCIF antibody
      • OPG antibody
      • Osteoclastogenesis inhibitory factor antibody
      • Osteoprotegerin antibody
      • PDB5 antibody
      • TNF receptor superfamily member 11b antibody
      • TNFRSF 11B antibody
      • TNFRSF11B antibody
      • TR 1 antibody
      • TR1 antibody
      • TR11B_HUMAN antibody
      • Tumor necrosis factor receptor superfamily member 11B antibody
      see all

    Anti-Osteoprotegerin antibody [EPR3592] images

    • ab124820 at 1/100 dilution, staining TNFRSF11B in Formalin fixed Paraffin-embedded Human kidney tissue by Immunohistochemistry.
    • ab124820 at 1/100 dilution, staining TNFRSF11B in Formalin fixed Paraffin-embedded Human heart tissue by Immunohistochemistry.
    • ab124820, at 1/100 dilution, staining TNFRSF11B in HeLa cells.

    References for Anti-Osteoprotegerin antibody [EPR3592] (ab124820)

    This product has been referenced in:
    • Ramos YF  et al. A gain of function mutation in TNFRSF11B encoding osteoprotegerin causes osteoarthritis with chondrocalcinosis. Ann Rheum Dis N/A:N/A (2014). Read more (PubMed: 24743232) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry
    Sample Human Cultured Cells (MCF-7)
    Specification MCF-7
    Permeabilization No
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Aug 16 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
    Sample Human Tissue sections (Breast carcinoma)
    Specification Breast carcinoma
    Permeabilization No
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Aug 16 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"