Overview

  • Product nameAnti-OSTM1 antibody
    See all OSTM1 primary antibodies
  • Description
    Rabbit polyclonal to OSTM1
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen, corresponding to amino acids 225-278 (REAYKTLSSL YSEMQKMNEL ENKAEPGTHL CIDVEDAMNI TRKLWSRTFN CSVP) of Human OSTM1.

  • Positive control
    • Human liver tissue; Transfected HEK293T cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab121327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.
WB 1/250 - 1/500.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionRequired for osteoclast and melanocyte maturation and function.
  • Involvement in diseaseDefects in OSTM1 are the cause of osteopetrosis autosomal recessive type 5 (OPTB5) [MIM:259720]; also called infantile malignant osteopetrosis 3. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. OPTB5 patients manifest primary central nervous system involvement in addition to the classical stigmata of severe bone sclerosis, growth failure, anemia, thrombocytopenia and visual impairment with optic atrophy.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chloride channel 7 beta subunit antibody
    • GAIP-interacting protein N terminus antibody
    • GIPN antibody
    • GL antibody
    • Grey lethal osteopetrosis antibody
    • HSPC019 antibody
    • OPTB5 antibody
    • Osteopetrosis-associated transmembrane protein 1 antibody
    • Ostm1 antibody
    • OSTM1_HUMAN antibody
    • OTTHUMP00000016938 antibody
    • OTTHUMP00000196342 antibody
    see all

Anti-OSTM1 antibody images

  • Immunocytochemistry/immunofluorescence analysis of A431 cells labelling OSTM1 with ab121327 shows positive staining in the nucleus, cytoplasm and vesicles.
  • All lanes : Anti-OSTM1 antibody (ab121327) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : OSTM1-transfected HEK293T over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))

    Developed using the ECL technique
  • ab121327, at 1/200, staining OSTM1 in paraffin embedded Human liver tissue by Immunohistochemistry.

References for Anti-OSTM1 antibody (ab121327)

ab121327 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"