Anti-Otoferlin antibody [13A9] (ab53233)
Key features and details
- Mouse monoclonal [13A9] to Otoferlin
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-Otoferlin antibody [13A9]
See all Otoferlin primary antibodies -
Description
Mouse monoclonal [13A9] to Otoferlin -
Host species
Mouse -
Specificity
This antibody reacts specifically with human Otoferlin protein (220 kDa). -
Tested applications
Suitable for: Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Fusion protein corresponding to Human Otoferlin aa 1-400.
Database link: Q9HC10 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 1.21% Tris, 0.75% Glycine, 2% Sucrose -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
13A9 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53233 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF | (1) |
1/100 - 1/500.
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Notes |
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Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/100 - 1/500. |
Target
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Function
Key calcium ion sensor involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion and in the control of neurotransmitter release at these output synapses. Interacts in a calcium-dependent manner to the presynaptic SNARE proteins at ribbon synapses of cochlear inner hair cells (IHCs) to trigger exocytosis of neurotransmitter. Also essential to synaptic exocytosis in immature outer hair cells (OHCs). May also play a role within the recycling of endosomes. -
Tissue specificity
Isoform 1 and isoform 3 are found in adult brain. Isoform 2 is expressed in the fetus and in adult brain, heart, placenta, skeletal muscle and kidney. -
Involvement in disease
Defects in OTOF are the cause of deafness autosomal recessive type 9 (DFNB9) [MIM:601071]. DFNB9 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
Defects in OTOF are a cause of non-syndromic auditory neuropathy autosomal recessive (NSRAN) [MIM:601071]. NSRAN is a form of sensorineural hearing impairment with absent or severely abnormal auditory brainstem response but normal otoacoustic emissions. Auditory neuropathies result from a lesion in the area including the inner hair cells, connections between the inner hair cells and the cochlear branch of the auditory nerve, the auditory nerve itself and auditory pathways of the brainstem. In some cases NSRAN phenotype can be temperature sensitive. -
Sequence similarities
Belongs to the ferlin family.
Contains 4 C2 domains. -
Cellular localization
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Basolateral cell membrane. Endoplasmic reticulum membrane. Cell membrane. Detected at basolateral cell membrane with synaptic vesicles surrounding the ribbon and at the presynaptic plasma membrane in the inner hair cells (IHCs). Colocalizes with GPR25 and RAB8B in inner hair cells. - Information by UniProt
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Database links
- Entrez Gene: 9381 Human
- Entrez Gene: 83762 Mouse
- Omim: 603681 Human
- SwissProt: Q9HC10 Human
- SwissProt: Q9ESF1 Mouse
- Unigene: 91608 Human
- Unigene: 244502 Mouse
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Alternative names
- AUNB1 antibody
- Deafness, autosomal recessive 9 antibody
- DFNB6 antibody
see all
Images
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Overlay histogram showing SHSY-5Y cells stained with ab53233 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53233, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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ICC/IF analysis of mouse inner ear tissue labeling Otoferlin with ab181781. At 30 days postnatal, Otoferlin is detected in the hair cells of the crista ampullaris (CA) , utricular macula (UM) and saccularmacula (SM).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (33)
ab53233 has been referenced in 33 publications.
- Ikäheimo K et al. MANF supports the inner hair cell synapse and the outer hair cell stereocilia bundle in the cochlea. Life Sci Alliance 5:N/A (2022). PubMed: 34815294
- Guo R et al. Complete Elimination of Peripheral Auditory Input Before Onset of Hearing Causes Long-Lasting Impaired Social Memory in Mice. Front Neurosci 15:723658 (2021). PubMed: 34385906
- Wang G et al. Mosaic CRISPR-stop enables rapid phenotyping of nonsense mutations in essential genes. Development 148:N/A (2021). PubMed: 33558388
- Hosoya M et al. Neuronal development in the cochlea of a nonhuman primate model, the common marmoset. Dev Neurobiol 81:905-938 (2021). PubMed: 34545999
- Kroll J et al. AP180 promotes release site clearance and clathrin-dependent vesicle reformation in mouse cochlear inner hair cells. J Cell Sci 133:N/A (2020). PubMed: 31843760