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Tagged fusion protein, corresponding to amino acids 1-395 of Human Otoferlin.
Our Abpromise guarantee covers the use of ab53233 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 23650376|
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|IHC-FoFr||1/50. PubMed: 19417007|
|WB||1/200 - 1/1000. Predicted molecular weight: 227 kDa.|
|IP||1/50 - 1/200.|
|ICC/IF||1/100 - 1/500.|
Immunohistochemistry analysis of mouse inner ear tissue labeling Otoferlin with ab181781. At 30 days postnatal, Otoferlin is detected in the hair cells of the crista ampullaris (CA) , utricular macula (UM) and saccularmacula (SM).