Overview

  • Product nameAnti-Otoferlin antibody [13A9]
  • Description
    Mouse monoclonal [13A9] to Otoferlin
  • SpecificityThis antibody reacts specifically with human Otoferlin protein (220 kDa).
  • Tested applicationsSuitable for: IHC-Fr, Flow Cyt, IHC-FoFr, WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Tagged fusion protein, corresponding to amino acids 1-395 of Human Otoferlin.

Properties

Applications

Our Abpromise guarantee covers the use of ab53233 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 23650376
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-FoFr 1/50. PubMed: 19417007
WB 1/200 - 1/1000. Predicted molecular weight: 227 kDa.
IP 1/50 - 1/200.
ICC/IF 1/100 - 1/500.

Target

  • FunctionKey calcium ion sensor involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion and in the control of neurotransmitter release at these output synapses. Interacts in a calcium-dependent manner to the presynaptic SNARE proteins at ribbon synapses of cochlear inner hair cells (IHCs) to trigger exocytosis of neurotransmitter. Also essential to synaptic exocytosis in immature outer hair cells (OHCs). May also play a role within the recycling of endosomes.
  • Tissue specificityIsoform 1 and isoform 3 are found in adult brain. Isoform 2 is expressed in the fetus and in adult brain, heart, placenta, skeletal muscle and kidney.
  • Involvement in diseaseDefects in OTOF are the cause of deafness autosomal recessive type 9 (DFNB9) [MIM:601071]. DFNB9 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
    Defects in OTOF are a cause of non-syndromic auditory neuropathy autosomal recessive (NSRAN) [MIM:601071]. NSRAN is a form of sensorineural hearing impairment with absent or severely abnormal auditory brainstem response but normal otoacoustic emissions. Auditory neuropathies result from a lesion in the area including the inner hair cells, connections between the inner hair cells and the cochlear branch of the auditory nerve, the auditory nerve itself and auditory pathways of the brainstem. In some cases NSRAN phenotype can be temperature sensitive.
  • Sequence similaritiesBelongs to the ferlin family.
    Contains 4 C2 domains.
  • Cellular localizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Basolateral cell membrane. Endoplasmic reticulum membrane. Cell membrane. Detected at basolateral cell membrane with synaptic vesicles surrounding the ribbon and at the presynaptic plasma membrane in the inner hair cells (IHCs). Colocalizes with GPR25 and RAB8B in inner hair cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • AUNB1 antibody
    • Deafness, autosomal recessive 9 antibody
    • DFNB6 antibody
    • DFNB9 antibody
    • Fer 1 like protein 2 antibody
    • Fer-1-like protein 2 antibody
    • FER1L2 antibody
    • NSRD9 antibody
    • Otof antibody
    • OTOF_HUMAN antibody
    • Otoferlin antibody
    see all

Anti-Otoferlin antibody [13A9] images

  • Overlay histogram showing SHSY-5Y cells stained with ab53233 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53233, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunohistochemistry analysis of mouse inner ear tissue labeling Otoferlin with ab181781. At 30 days postnatal, Otoferlin is detected in the hair cells of the crista ampullaris (CA) , utricular macula (UM) and saccularmacula (SM).

References for Anti-Otoferlin antibody [13A9] (ab53233)

This product has been referenced in:
  • Neef J  et al. Modes and regulation of endocytic membrane retrieval in mouse auditory hair cells. J Neurosci 34:705-16 (2014). IHC (PFA fixed) ; Mouse . Read more (PubMed: 24431429) »
  • Liu Z  et al. In vivo generation of immature inner hair cells in neonatal mouse cochleae by ectopic Atoh1 expression. PLoS One 9:e89377 (2014). IF ; Mouse . Read more (PubMed: 24586731) »

See all 11 Publications for this product

Product Wall

Application IHC - Wholemount
Sample (Paddlefish) Polyodon spathula Tissue (hair cells in otic vesicle)
Specification hair cells in otic vesicle
Username

Abcam user community

Verified customer

Submitted Jun 22 2014

Application Immunocytochemistry/ Immunofluorescence
Sample Zebrafish Cell (Whole organism)
Specification Whole organism
Fixative Paraformaldehyde
Permeabilization Yes - triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Colin Johnson

Verified customer

Submitted Jun 20 2012

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Expiration date: dd mm 2012

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Thank you for your inquiry. The immunogen this antibody was raised against is a tagged fusion protein, corresponding to amino acids 1-395 of Human Otoferlin. (Immunogen section on the datasheet). We are not aware that the epitope that is recogn...

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