Overview

  • Product name
    Anti-OTOL1 antibody
  • Description
    Rabbit polyclonal to OTOL1
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: SYHITVRGRP ARISLVAQNK KQFKSRETLY GQEIDQASLL VILKLSAGDQ VWLEVSKDWN GVYVSAEDDS I, corresponding to amino acids 391-461 of Human OTOL1.

  • Positive control
    • Human colon tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab122687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Sequence similarities
    Belongs to the OTOL1 family.
    Contains 1 C1q domain.
    Contains 3 collagen-like domains.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C1q and tumor necrosis factor related protein 15 antibody
    • C1QTNF15 antibody
    • Otol1 antibody
    • OTOL1_HUMAN antibody
    • Otolin 1 homolog antibody
    • Otolin-1 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and golgi apparatus. Recommended concentration of ab122687 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122687, at 1/200 dilution, staining OTOL1 in paraffin-embedded Human colon tissue by Immunohistochemistry.

References

ab122687 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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