Overview

  • Product nameAnti-OTUB1 antibody
    See all OTUB1 primary antibodies
  • Description
    Goat polyclonal to OTUB1
  • Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Cynomolgus Monkey
  • Immunogen

    Synthetic peptide: C-

    YKEYAEDDNIYQQK

    corresponding to internal sequence amino acids 58-71 of human OTUB1 (NP_060140.2).

  • Positive control
    • Human brain (cerebellum) and, mouse and rat brain lysates.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab82154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
ELISA
  • Application notesPeptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/64000.
    WB: Use at a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 31 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionHydrolase that can remove conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform 1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 stabilizes RNF128 and promotes anergy. Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). Mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. Not able to cleave di-ubiquitin. Also capable of removing NEDD8 from NEDD8 conjugates, but with a nuch lower preference compared to 'Lys-48'-linked ubiquitin.
    • Tissue specificityIsoform 1 is ubiquitous. Isoform 2 is expressed only in lymphoid tissues such as tonsils, lymph nodes and spleen, as well as peripheral blood mononuclear cells.
    • Sequence similaritiesBelongs to the peptidase C65 family.
      Contains 1 OTU domain.
    • DomainIn addition to ubiquitin-binding at the Cys-91 active site, a proximal ubiquitin-binding site is also present at Cys-23 Occupancy of the active site is needed to enable tight binding to the second site. Distinct binding sites for the ubiquitins may allow to discriminate among different isopeptide linkages (i.e. 'Lys-48'-, 'Lys-63'-linked polyubiquitin) in polyubiquitin substrates and achieve linkage-specific deubiquitination.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Deubiquitinating enzyme OTUB1 antibody
      • hOTU1 antibody
      • HSPC263 antibody
      • OTB1 antibody
      • OTU domain containing ubiquitin aldehyde binding protein 1 antibody
      • OTU domain, ubiquitin aldehyde binding 1 antibody
      • OTU domain-containing Ubal-binding protein 1 antibody
      • OTU domain-containing ubiquitin aldehyde-binding protein 1 antibody
      • OTU-domain Ubal-binding 1 antibody
      • OTU1 antibody
      • Otub1 antibody
      • OTUB1_HUMAN antibody
      • Otubain 1 antibody
      • Otubain-1 antibody
      • Ubiquitin specific processing protease OTUB1 antibody
      • Ubiquitin thioesterase OTUB1 antibody
      • ubiquitin-specific protease otubain 1 antibody
      • Ubiquitin-specific-processing protease OTUB1 antibody
      see all

    Anti-OTUB1 antibody images


    • Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 31 kDa


      Exposure time : 1 minute

      Image courtesy of an Anonymous review

      Anti-OTUB1 antibody (ab82154) reactivity with reduced HEK293 cell lysate after transient transfection (48h) of control siRNA (lane 1-3) or siRNA targeting OTUB1 (lane 4 and 5). After SDS-PAGE, membranes were blocked in 5% milk TBS + 0.1% Tween for 1h at 25°C before incubation with ab82154 (1:1000 dilution in 5% milk TBS + 0.1% Tween) for 16h at 4ºC. Blots was then incubated with an anti-Goat HRP-conjugated secondary antibody before developing with ECL. Anti-tubulin blot included as a loading control. 

      See Abreview

    • Anti-OTUB1 antibody (ab82154) at 0.5 µg/ml + mouse brain lysate in RIPA buffer at 35 µg
      Developed using the ECL technique

      Predicted band size : 31 kDa
      Observed band size : 31 kDa


      Exposure time : 1 hour

    References for Anti-OTUB1 antibody (ab82154)

    ab82154 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
    Sample Human Cell (HaCaT)
    Specification HaCaT
    Permeabilization Yes - Triton X-100 plus
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Oct 14 2013

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (10)
    Sample Mouse Cell lysate - whole cell (NMuMG cells)
    Specification NMuMG cells
    Treatment control or OTUB1 siRNA (300pM per 10 cm dish, 48h)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Ms. Lina Herhaus

    Verified customer

    Submitted Oct 11 2013

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (10%)
    Sample Human Cell lysate - whole cell (HEK293)
    Specification HEK293
    Treatment control or OTUB1 siRNA (300pM per 10 cm dish, 48h)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 11 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"