Overview

  • Product nameAnti-OTUB2 antibody
    See all OTUB2 primary antibodies
  • Description
    Rabbit polyclonal to OTUB2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from an internal region of human OTUB2.

  • Positive control
    • Extracts from HepG2, Jurkat, COLO 205, HT-29 and 293 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab74371 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
ELISA 1/20000.

Target

  • FunctionHydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains.
  • Tissue specificityWidely expressed. Expressed at higher level in brain.
  • Sequence similaritiesBelongs to the peptidase C65 family.
    Contains 1 OTU domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • C14orf137 antibody
    • Deubiquitinating enzyme OTUB2 antibody
    • FLJ21916 antibody
    • MGC3102 antibody
    • OTB2 antibody
    • OTU domain containing ubiquitin aldehyde binding protein 2 antibody
    • OTU domain, ubiquitin aldehyde binding 2 antibody
    • OTU domain-containing ubiquitin aldehyde-binding protein 2 antibody
    • OTU2 antibody
    • Otub2 antibody
    • OTUB2_HUMAN antibody
    • Otubain 2 antibody
    • Otubain-2 antibody
    • Ubiquitin specific processing protease OTUB2 antibody
    • Ubiquitin specific protease otubain 2 antibody
    • Ubiquitin thioesterase OTUB2 antibody
    • Ubiquitin-specific-processing protease OTUB2 antibody
    see all

Anti-OTUB2 antibody images

  • All lanes : Anti-OTUB2 antibody (ab74371) at 1/500 dilution

    Lane 1 : Extract from HepG2 cells
    Lane 2 : Extract from Jurkat cells
    Lane 3 : Extract from COLO 205 cells
    Lane 4 : Extract from HT-29 cells
    Lane 5 : Extract from 293 cells
    Lane 6 : Extract from COLO 205 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 27 kDa
    Observed band size : 27 kDa
    Additional bands at : 34 kDa. We are unsure as to the identity of these extra bands.

References for Anti-OTUB2 antibody (ab74371)

ab74371 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (U2OS)
Specification U2OS
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Carlos le Sage

Verified customer

Submitted Oct 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"