The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesPeptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1:16000.
WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 27 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionHydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains.
Tissue specificityWidely expressed. Expressed at higher level in brain.
Sequence similaritiesBelongs to the peptidase C65 family. Contains 1 OTU domain.