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Family with sequence similarity 105, member B antibody
OTU deubiquitinase with linear linkage specificity antibody
Protein FAM105B antibody
Ubiquitin thioesterase Gumby antibody
Western blot - Anti-OTULIN antibody (ab114137)
All lanes : Anti-OTULIN antibody (ab114137) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 40 kDa Observed band size : 40 kDa Additional bands at : 73 kDa (possible non-specific binding).
Exposure time : 12 minutes
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab114137 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.