Products:Neuroscience >> Sensory System >> Visual system
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ab24347 |
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ab24347 |
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Read our guarantee »Publishing research using ab21990? Please let us know so that we can cite the reference in this datasheet
ab21990 has been referenced in 18 publications.
Publishing research using ab21990? Please let us know so that we can cite the reference in this datasheet
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Mouse E6 embryos were sectioned and then fixed in paraformaldehyde. Antigen retrieval was performed using citric acid and the sections permeablized and blocked for 1 hour in serum. Embryos were stained with ab21990 (1/2500) diluted in 10% serum, 1% ovalbumin in PBSTween for 14 hours at 4°C. They were then washed and stained with a goat anti-rabbit biotin conjugated antibody. After using a biotinylated secondary, ABC and then DAB was used for color development.
ab21990 staining Otx2 in embryonic day 14.5 (E14.5) mouse eye by Immunohistochemistry (formalin fixed, paraffin embedded sections).
The embryo was fixed in 10% neutral buffered formalin overnight at room temperature, then paraffin-embedded and sectioned at 4µm. Following deparaffinization and antigen retrieval in a rice steamer in 10mM sodium citrate (pH 6.0), the sections were blocked with 5% normal goat serum for half an hour at room temperature. ab21990 was diluted in PBS at 1/100, and incubated overnight at 4°C. The secondary was goat anti-rabbit Alexa®568, diluted in PBS with 1.5% normal goat serum. The mounting medium contained the nuclear stain DAPI (blue).
Image courtesy of Erin Bassett
All lanes : Anti-Otx1 + Otx2 antibody - ChIP Grade (ab21990) at 1 µg/ml
Lane 1 : Y79 Lysate
Lane 2 : Y79 Lysate with
Lysates/proteins at 20 µg per lane.
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size : 31.6 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Additional bands at : 28 kDa (possible cleavage fragment,isoform,non-specific binding).
The image shows staining of Otx2 in human embryonic stem cells differentiated into neuroectoderm. As would be expected, staining is nuclear.
L. Vallier, University of Cambridge
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