Anti-Otx1 + Otx2 antibody - ChIP Grade (ab21990)

I have only been using ab21990 the order confirmation number is .   Please go ahead and send me ab76748 as a replacement. Thank you for sending time on resolving these issues.  

ANSWER:

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number .

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Here is the link to ab92515 ( http://www.abcam.com/Otx2-antibody-EPR3347-ab92515.html) The western has a single band close to the 25kD marker band.    While the ab21990 has two bands one just above 25kD and one at 37kD. Since 21990 detects both OTX1 (37kD) and OTX2 (32kD) I assume my lower band is OTX2. But if ab92515 detects both proteins then why don’t we see two bands?   Whereas ab76748 that you suggested previously doesn’t have any western images in the datasheet.   I am trying to estimate amounts of OTX2 only. And this is important hence all the questions.  

ANSWER:

Thank you for getting back to me with these questions.

I have been under the assumption that you havwe been using ab21190 for your experiments, have you been using ab92515 as well?Based on sequence homology ab92515 will recognize both proteins. The absence of a second band in the image is unresolved but may be due to poor separation or extrememly low levels of Otx1 in the sample.

Using ab21190 the lower band will be Otx2 which, in either isoform, has a lower molecular weight that Otx1. (http://www.uniprot.org/uniprot/P32243&http://www.uniprot.org/uniprot/P32242).

Although ab76748 does not have any western blot images in the datasheet we have been assured that the immunogen used for this has no cross reactivity to Otx1.This product is guaranteed to work in western bloton ​human tissues and is covered by our Abpromise for a period of six months. Since we have been unable to resolve this problem, if you have purchased this product within the last 180 days, I would be happy to either send this replacement antibody or to process a refund. If you could please supply me with your Abcam order confirmation number for the purchase of ab21190 I can begin to process this replacement,

How about ab92515? Is there a reason you have not mention this Antibody – except that it is monoclonal and the others polyclonal? Does the immunogen cross react ? It has images that seem to indicate specificity to OTX2 only,  in a western.  

ANSWER:

Thank you for contacting us.

The immunogen for ab92515 shares 100% identity with OTX1, so cross reactivity is predicted.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Phoned because the target of ab21990 has changed and is now unsure about their data.

ANSWER:

Thank you for contacting us.

I am sorry that you have found this product unsatisfactory following its recent target refinement. As we discussed on the phone the sequence for ab21990 is shared by both OTX1 and OTX2. It was because of this that we have changed the targets.

We have other products which has been guaranteed to work in WB and ICH on human tissue. One such product is ab76748. According to information that I have recieved form the lab, this antibody is specific to Otx2 only with no cross reactivity with Otx1. The immunogenic peptide was selected from specific domains of Otx2.


Another product I would like to mention is ab92326. The immunogen for this OTX2 antibody shares 81% identity with OTX1, so the antibody is not predicted to cross-react with OTX1. However, it is not guaranteed without testing

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for informing me of the problem with the Otx2 polyclonal antibody (Ab21990). I have used this antibody and failed to get the

expected results and so I would be grateful to try a different lot; here is my original order number:1736952-1.

Kind regards,

ANSWER:

Thank you for contacting us.

We have shipped lot GR9703-1 in your order last time; we still have this lot in stock and our retesting results are quite promising. I am sorry that the product failed in your experiment. I would like to know more about the experiment you have performed. Could you provide the following information? - Which application the antibody was used in? - How do you describe the problem? -Could you attach a brief protocol and any information regarding troubleshooting steps?

I will look forward to hearing from you soon.