Overview

  • Product nameAnti-Otx1 antibody
    See all Otx1 primary antibodies
  • Description
    Rabbit polyclonal to Otx1
  • SpecificityWe have not been able to test Rabbit polyclonal to Otx1 (ab25985) in Western Blot due to difficulties in accessing the appropriate lysates. We would appreciate any feedback on this antibody.
  • Tested applicationsSuitable for: IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chimpanzee, Gorilla, Catfish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Mouse Otx1.

  • Positive control
    • This antibody gave a positive signal in the following tissues: Formalin Fixed Paraffin Embedded Mouse E11.5 Embryo Normal; Formalin Fixed Paraffin Embedded Mouse E17 Brain Normal.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
IHC-Fr 1/100.

Target

Anti-Otx1 antibody images

  • Anti-Otx1 antibody (ab25985) at 1 µg/ml + OTX1 (Human) - Recombinant Protein at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab25985 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

    Ab25985 recognizes the full length tagged recombinant protein which has an expected molecular weight of 75 kDa.

  • IHC-P image of Otx1 antibody (ab25985) on E11.5 mouse embryo sections. The sections were fixed in Paraformaldehyde and underwent heat mediated antigen retrieval using EDTA. These sections were then blocked in serum for 1 hour, before the Otx1 antibody was added at 1:100 dilution and left overnight at 4oC.

    See Abreview

References for Anti-Otx1 antibody (ab25985)

This product has been referenced in:
  • Saito T  et al. Neocortical Layer Formation of Human Developing Brains and Lissencephalies: Consideration of Layer-Specific Marker Expression. Cereb Cortex : (2010). Read more (PubMed: 20624841) »
  • Hadjivassiliou G  et al. The application of cortical layer markers in the evaluation of cortical dysplasias in epilepsy. Acta Neuropathol : (2010). IHC-P ; Human . Read more (PubMed: 20411268) »

See all 2 Publications for this product

Product Wall

This antibody is guaranteed to work in human and in Immunohistochemistry on paraffinised tissue (IHC-P) so yes this antibody is suitable for use in your experiment. However, with IHC-P an enzymatic detection is usually preferable over an immunofluorese...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (tonsil, colon cancer)
Specification tonsil, colon cancer
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 20 min, 100C
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Nov 02 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (E11.5 Embryo)
Specification E11.5 Embryo
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA
Permeabilization Yes - 0.5% Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20%
Username

Abcam user community

Verified customer

Submitted Aug 05 2011

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mouse neocortex E14)
Specification Mouse neocortex E14
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization Yes - 0.025% TX100
Blocking step (agent) for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Jul 23 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (E12.5 Whole brain section)
Specification E12.5 Whole brain section
Fixative Paraformaldehyde
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5%
Username

Dr. Dean Griffiths

Verified customer

Submitted Nov 30 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"