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Products:Neuroscience >> Sensory System >> Visual system
Overview
Product name
Anti-Otx1 + Otx2 antibody - ChIP Grade
Description
Rabbit polyclonal to Otx1 + Otx2 - ChIP Grade
Tested applications
IHC-Fr, ICC/IF, ChIP, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Chicken, Cow, Human, Xenopus laevis
Predicted to work with
Zebrafish
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human Otx2.
(Peptide available as ab24347.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Organogenesis >> Nervous system development
Developmental Biology >> Lineage specification >> Ectoderm
Developmental Biology >> Embryogenesis >> Axis formation and symmetry
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Stem Cells >> Lineage Markers >> Ectoderm
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Sensory System >> Visual system
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx1 + Otx2 antibody - ChIP Grade (ab21990)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx1 + Otx2 antibody - ChIP Grade (ab21990)
(enlarge)
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Western blot - Anti-Otx1 + Otx2 antibody - ChIP Grade (ab21990)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab21990 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use a concentration of 0.8 µg/ml
ICC/IF: Use a concentration of 0.8 - 4 µg/ml.
ChIP: Use at an assay dependent dilution. (PubMed: 18057103)
WB: Use a concentration of 1 µg/mlDetects a band of approximately 37 kDa (predicted molecular weight: 31.6 kDa).Can be blocked with Otx2 peptide (ab24347).
IHC-P: Use at an assay dependent dilution. (PubMed: 20150232)
Target
Relevance
Function: Probably plays a role in the development of the brain and the sense organs. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'.
Tissue specificity: Expressed in brain.
Disease: Defects in OTX2 are the cause of microphthalmia syndromic type 5 (MCOPS5) [MIM:610125]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Up to 80% of cases of microphthalia occur in association with syndromes that include non-ocular abnormalities. MCOPS5 patients manifest unilateral or bilateral microphthalmia/clinical anophthalmia and variable additional features including coloboma, microcornea, cataract, retinal dystrophy, hypoplasia or agenesis of the optic nerve, agenesis of the corpus callosum, developmental delay, joint laxity, hypotonia, and seizures.
Similarity: Belongs to the paired homeobox family. Bicoid subfamily. Contains 1 homeobox DNA-binding domain.
Developmental stage: Embryo.
Alternative names
- FLJ38361 antibody
- Homeobox protein OTX 1 antibody
- Homeobox protein OTX1 antibody
see all
Database links
- Entrez Gene: 5013 Human
- Entrez Gene: 5015 Human
- Entrez Gene: 18423 Mouse
- Entrez Gene: 30501 Zebrafish
- SwissProt: P32242 Human
- SwissProt: P32243 Human
- SwissProt: P80205 Mouse
- SwissProt: P80206 Mouse
see all
Anti-Otx1 + Otx2 antibody - ChIP Grade images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx1 + Otx2 antibody - ChIP Grade (ab21990)
Mouse E6 embryos were sectioned and then fixed in paraformaldehyde. Antigen retrieval was performed using citric acid and the sections permeablized and blocked for 1 hour in serum. Embryos were stained with ab21990 (1/2500) diluted in 10% serum, 1% ovalbumin in PBSTween for 14 hours at 4°C. They were then washed and stained with a goat anti-rabbit biotin conjugated antibody. After using a biotinylated secondary, ABC and then DAB was used for color development.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx1 + Otx2 antibody - ChIP Grade (ab21990)
ab21990 staining Otx2 in embryonic day 14.5 (E14.5) mouse eye by Immunohistochemistry (formalin fixed, paraffin embedded sections).
The embryo was fixed in 10% neutral buffered formalin overnight at room temperature, then paraffin-embedded and sectioned at 4µm. Following deparaffinization and antigen retrieval in a rice steamer in 10mM sodium citrate (pH 6.0), the sections were blocked with 5% normal goat serum for half an hour at room temperature. ab21990 was diluted in PBS at 1/100, and incubated overnight at 4°C. The secondary was goat anti-rabbit Alexa®568, diluted in PBS with 1.5% normal goat serum. The mounting medium contained the nuclear stain DAPI (blue).
Image courtesy of Erin Bassett
Western blot - Anti-Otx1 + Otx2 antibody - ChIP Grade (ab21990)
All lanes : Anti-Otx1 + Otx2 antibody - ChIP Grade (ab21990) at 1 µg/ml
Lane 1 : Y79 Lysate
Lane 2 : Y79 Lysate with Otx2 peptide (ab24347) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size : 31.6 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Additional bands at : 28 kDa (possible cleavage fragment,isoform,non-specific binding).
Immunocytochemistry/ Immunofluorescence - Anti-Otx1 + Otx2 antibody - ChIP Grade (ab21990)
The image shows staining of Otx2 in human embryonic stem cells differentiated into neuroectoderm. As would be expected, staining is nuclear.
L. Vallier, University of Cambridge
References for Anti-Otx1 + Otx2 antibody - ChIP Grade (ab21990)
This product has been referenced in:
- Kameda Yet al. Hes1 regulates the number and anterior-posterior patterning of mesencephalic dopaminergic neurons at the mid/hindbrain boundary (isthmus). Dev Biol : (2011). IHC-P; Mouse.Read more (PubMed: 21798254) »
- Greber Bet al. FGF signalling inhibits neural induction in human embryonic stem cells. EMBO J 30:4874-84 (2011).Read more (PubMed: 22085933) »
See all 17 publications for this product
Publishing research using ab21990? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"