Anti-Otx1 + Otx2 antibody [EPR3347] (ab92515)


  • Product nameAnti-Otx1 + Otx2 antibody [EPR3347]
    See all Otx1 + Otx2 primary antibodies
  • Description
    Rabbit monoclonal [EPR3347] to Otx1 + Otx2
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    This information is considered to be commercially sensitive.

  • Positive control
    • This antibody gave a positive signal in the following lysates: Y79 whole cell; Human Fetal Brain tissue; Otx1 recombinant protein.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


Associated products


Our Abpromise guarantee covers the use of ab92515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 32 kDa.
IHC-P 1/100 - 1/250.


  • Relevance

    Function: Probably plays a role in the development of the brain and the sense organs. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'.

    Tissue specificity: Expressed in brain.

    Disease: Defects in OTX2 are the cause of microphthalmia syndromic type 5 (MCOPS5) [MIM:610125]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Up to 80% of cases of microphthalia occur in association with syndromes that include non-ocular abnormalities. MCOPS5 patients manifest unilateral or bilateral microphthalmia/clinical anophthalmia and variable additional features including coloboma, microcornea, cataract, retinal dystrophy, hypoplasia or agenesis of the optic nerve, agenesis of the corpus callosum, developmental delay, joint laxity, hypotonia, and seizures.

    Similarity: Belongs to the paired homeobox family. Bicoid subfamily. Contains 1 homeobox DNA-binding domain.

    Developmental stage: Embryo.

  • Database links
  • Alternative names
    • FLJ38361 antibody
    • Homeobox protein OTX 1 antibody
    • Homeobox protein OTX1 antibody
    • Homeobox protein OTX2 antibody
    • MCOPS 5 antibody
    • MCOPS5 antibody
    • MGC15736 antibody
    • MGC45000 antibody
    • Orthodenticle 1 antibody
    • Orthodenticle 2 antibody
    • Orthodenticle homeobox 1 antibody
    • Orthodenticle homeobox 2 antibody
    • Orthodenticle homolog 1 antibody
    • Orthodenticle homolog 2 (Drosophila) antibody
    • Orthodenticle homolog 2 antibody
    • Orthodenticle1 antibody
    • Orthodenticle2 antibody
    • Otx 1 antibody
    • Otx 2 antibody
    • otx1 antibody
    • OTX1_HUMAN antibody
    • otx2 antibody
    • OTX2_HUMAN antibody
    see all

Anti-Otx1 + Otx2 antibody [EPR3347] images

  • Anti-Otx1 + Otx2 antibody [EPR3347] (ab92515) at 1/2500 dilution (purified) + Y79 cell lysate at 10 µg

    HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

    Predicted band size : 32 kDa
    Observed band size : 32 kDa

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemical staining of paraffin embedded human cerebral cortex with purified ab92515 at a working dilution of 1/250. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Anti-Otx1 + Otx2 antibody [EPR3347] (ab92515) at 1/1000 dilution (unpurified) + Y79 cell lysate at 10 µg

    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 32 kDa
  • Anti-Otx1 + Otx2 antibody [EPR3347] (ab92515) at 1 µg/ml (unpurified) + OTX1 Recombinant Protein at 0.1 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 32 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • Paraffin embedded Human brain tissue labelled with unpurified ab92515 at 1/100 dilution.

References for Anti-Otx1 + Otx2 antibody [EPR3347] (ab92515)

ab92515 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

Our antibodies are tested in WB under reducing and denaturing conditions unless specified otherwise on the datasheet. So for this antibody I would recomend reducing/denaturing conditions as it has not been tested...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Thank you for getting back to me with these questions.

I have been under the assumption that you havwe been using ab21190 for your experiments, have you been using ab92515 as well?Based on sequence homology ab92515 will recognize both protei...

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Thank you for contacting us.

The immunogen for ab92515 shares 100% identity with OTX1, so cross reactivity is predicted.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any mor...

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