Overview

  • Product nameAnti-Ovalbumin antibody [1E7]
    See all Ovalbumin primary antibodies
  • Description
    Mouse monoclonal [1E7] to Ovalbumin
  • SpecificityAb17291 reacts with ovalbumin in denatured or modified forms (native Ovalbumin surface-adsorbed, heat-denatured or reduced).
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Chicken
  • Immunogen

    Full length native protein (purified) corresponding to Chicken Ovalbumin. Full length native protein (purified), isolated from chicken egg white formaldehyde and lysine treated for 2 weeks at 35°C and then heat denatured by autoclaving for 1 h at 110°C.

  • Positive control
    • ELISA: recombinant protein

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Preservative: 0.1% Sodium azide
    Constituents: 0.0268% PBS, 2.9% Sodium chloride
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesFemale CF1 x BALB/c mice were immunized i.p. with immunogen adsorbed onto Al(OH)3.
  • ClonalityMonoclonal
  • Clone number1E7
  • IsotypeIgG2b
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/8000. A strong reaction is seen in ELISA, with ovalbumin directly coated onto the microtiter well (1ug/ml) (See Koch et al reference).
WB 1/2000. Detects a band of approximately 45 kDa.

Target

  • FunctionStorage protein of egg white. Lack protease inhibitory activity.
  • Tissue specificityMajor protein of egg white.
  • Sequence similaritiesBelongs to the serpin family. Ov-serpin subfamily.
  • Post-translational
    modifications
    The signal sequence is not cleaved. The functional signal for membrane translocation of ovalbumin becomes accessible when the nascent chain is 50 to 60 residues long. The hydrophobic sequence which lies between residues 27 and 43 folds back on the preceding residues to form an amphipathic hairpin structure which is the signal element recognized by the membrane.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Allergen Gal d II antibody
    • Egg albumin antibody
    • OVAL_CHICK antibody
    • Ovalbumin antibody
    • Plakalbumin antibody
    • Serpin peptidase inhibitor antibody
    • SERPINB14 antibody
    see all

References for Anti-Ovalbumin antibody [1E7] (ab17291)

This product has been referenced in:

See all 3 Publications for this product

Product Wall



The monoclonal anti-ovalbumin ab17291 and ab17293 are issued from 2 different clones and the immunogen used to raise them is the full length native protein. Unfortunately the epitopes have not been mapped.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"