Overview

  • Product name
    Anti-Ovalbumin antibody [1E7]
    See all Ovalbumin primary antibodies
  • Description
    Mouse monoclonal [1E7] to Ovalbumin
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Chicken
  • Immunogen

    Full length native protein (purified) corresponding to Chicken Ovalbumin. Full length native protein (purified), isolated from chicken egg white formaldehyde and lysine treated for 2 weeks at 35°C and then heat denatured by autoclaving for 1 h at 110°C.

  • Positive control
    • ELISA: recombinant protein

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituents: 0.0268% PBS, 2.9% Sodium chloride
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Female CF1 x BALB/c mice were immunized i.p. with immunogen adsorbed onto Al(OH)3.
  • Clonality
    Monoclonal
  • Clone number
    1E7
  • Isotype
    IgG2b
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/8000. A strong reaction is seen in ELISA, with ovalbumin directly coated onto the microtiter well (1ug/ml) (See Koch et al reference).
WB 1/2000. Detects a band of approximately 45 kDa.

Target

  • Function
    Storage protein of egg white. Lack protease inhibitory activity.
  • Tissue specificity
    Major protein of egg white.
  • Sequence similarities
    Belongs to the serpin family. Ov-serpin subfamily.
  • Post-translational
    modifications
    The signal sequence is not cleaved. The functional signal for membrane translocation of ovalbumin becomes accessible when the nascent chain is 50 to 60 residues long. The hydrophobic sequence which lies between residues 27 and 43 folds back on the preceding residues to form an amphipathic hairpin structure which is the signal element recognized by the membrane.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Allergen Gal d II antibody
    • Egg albumin antibody
    • OVAL_CHICK antibody
    • Ovalbumin antibody
    • Plakalbumin antibody
    • Serpin peptidase inhibitor antibody
    • SERPINB14 antibody
    see all

References

This product has been referenced in:

See all 3 Publications for this product

Customer reviews and Q&As



The monoclonal anti-ovalbumin ab17291 and ab17293 are issued from 2 different clones and the immunogen used to raise them is the full length native protein. Unfortunately the epitopes have not been mapped.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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