Overview

  • Product nameAnti-Ovalbumin antibody [6C8]
    See all Ovalbumin primary antibodies
  • Description
    Mouse monoclonal [6C8] to Ovalbumin
  • SpecificityThe antibody reacts strongly with Ovalbumin (OA) in denatured and modified forms (native OA surface-adsorbed, heat-denatured or reduced). Strong reaction is seen in ELISA, with OA directly coated onto the microtiterwell. In dotblotting it reacts with both native and denatured OA adsorbed onto nitrocellulose. In Western blotting after SDS-PAGE, the antibody reacts with OA in reduced as well as unreduced forms.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Chicken
  • Immunogen

    Full length native protein (purified) formaldehyde treated and heat denatured by incubation for 1 h at 105°C (Chicken).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.5M Sodium chloride, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesImmunized i.p. with immunogen adsorbed onto Al(OH)3.
  • ClonalityMonoclonal
  • Clone number6C8
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/4000.
WB 1/1000. Detects a band of approximately 45 kDa.

Target

  • FunctionStorage protein of egg white. Lack protease inhibitory activity.
  • Tissue specificityMajor protein of egg white.
  • Sequence similaritiesBelongs to the serpin family. Ov-serpin subfamily.
  • Post-translational
    modifications
    The signal sequence is not cleaved. The functional signal for membrane translocation of ovalbumin becomes accessible when the nascent chain is 50 to 60 residues long. The hydrophobic sequence which lies between residues 27 and 43 folds back on the preceding residues to form an amphipathic hairpin structure which is the signal element recognized by the membrane.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Allergen Gal d II antibody
    • Egg albumin antibody
    • OVAL_CHICK antibody
    • Ovalbumin antibody
    • Plakalbumin antibody
    • Serpin peptidase inhibitor antibody
    • SERPINB14 antibody
    see all

References for Anti-Ovalbumin antibody [6C8] (ab17293)

This product has been referenced in:
  • Grødeland G  et al. The Specificity of Targeted Vaccines for APC Surface Molecules Influences the Immune Response Phenotype. PLoS One 8:e80008 (2013). WB . Read more (PubMed: 24244595) »
  • Palmieri M  et al. Development of an analytical protocol for a fast, sensitive and specific protein recognition in paintings by enzyme-linked immunosorbent assay (ELISA). Anal Bioanal Chem 399:3011-23 (2011). ELISA . Read more (PubMed: 21170522) »

See all 4 Publications for this product

Product Wall

The two articles at the PubMed addresses below discuss the rationale for the preparation of the immunogen that generated the monoclonal antibody ab17293. The epitope that it detects (LEPINF, aa130-135) is listed in Table 1 of the 2014 paper, which refe...

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Thank you for contacting us.

There is just one lot available for ab17293. The lot number is: 1247369.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for your note.

This is to let you know that I have just contacted and asked our Account Department to raise a credit note for you - for the cost of one vial of ab17293. For your information, the internal reference note for this cred...

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Thank you for your message and for your updates.

I am very sorry about this news. Currently, we have the same batch in stock and we do not have new batch available for a while. I can offer a credit note which can be used for any products which...

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Thank you for your response.

This is to let you know that I have placed a new order for you - for one vial ofab17293 as a free of charge replacement and the new order number is 1083493.

I hope the second vial will work as it is expe...

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Thank you for your response.

We have not had any complaint about this antibody since 2008. According to our record, we have sold several vials of this batch without any problem. This implies that it is very unlikely that the problem is batch-...

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Thank you for your response.

I can offer you a new vial as free of charge replacement or a credit note which can be used in future purchase. The product will be from the same purification since currently we do not have a new batch.

...

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Thank you for your enquiry regarding ab17293 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

I would like to reassure you that our Abpromise a...

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Thank you for your inquiry.


We do recommend that you store ab17293 at +4C in the dark. Generally, antibodies stored at +4C are viable for up to a year. I wouldn't recommend freezing it. Since we haven't tested the antibody after it's be...

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Thank you for your inquiry. The immunogen for this antibody was the full length native and purified protein (chicken). The lab confirmed that the epitope that the antibody recognizes was not mapped. therefore I am unable to answer your ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"