Anti-Ovalbumin antibody [6C8] (ab17293)
Key features and details
- Mouse monoclonal [6C8] to Ovalbumin
- Suitable for: WB, ELISA
- Reacts with: Chicken
- Isotype: IgG1
Overview
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Product name
Anti-Ovalbumin antibody [6C8]
See all Ovalbumin primary antibodies -
Description
Mouse monoclonal [6C8] to Ovalbumin -
Host species
Mouse -
Specificity
The antibody reacts strongly with Ovalbumin (OA) in denatured and modified forms (native OA surface-adsorbed, heat-denatured or reduced). Strong reaction is seen in ELISA, with OA directly coated onto the microtiterwell. In dotblotting it reacts with both native and denatured OA adsorbed onto nitrocellulose. In Western blotting after SDS-PAGE, the antibody reacts with OA in reduced as well as unreduced forms. -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Chicken -
Immunogen
Full length native protein (purified) corresponding to Chicken Ovalbumin. Purified chicken ovalbumin was treated with formaldehyde and lysine for 2 weeks at 35°C and then autoclaved for 1 h at 110°C.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 2.9% Sodium chloride -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Immunized i.p. with immunogen adsorbed onto Al(OH)3. -
Clonality
Monoclonal -
Clone number
6C8 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab17293 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
Target
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Function
Storage protein of egg white. Lack protease inhibitory activity. -
Tissue specificity
Major protein of egg white. -
Sequence similarities
Belongs to the serpin family. Ov-serpin subfamily. -
Post-translational
modificationsThe signal sequence is not cleaved. The functional signal for membrane translocation of ovalbumin becomes accessible when the nascent chain is 50 to 60 residues long. The hydrophobic sequence which lies between residues 27 and 43 folds back on the preceding residues to form an amphipathic hairpin structure which is the signal element recognized by the membrane. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 396058 Chicken
- SwissProt: P01012 Chicken
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Alternative names
- Allergen Gal d II antibody
- Egg albumin antibody
- OVAL_CHICK antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab17293 has been referenced in 11 publications.
- Celus W et al. Plexin-A4 Mediates Cytotoxic T-cell Trafficking and Exclusion in Cancer. Cancer Immunol Res 10:126-141 (2022). PubMed: 34815265
- Chen CJ et al. Affordable IgY-based antiviral prophylaxis for resource-limited settings to address epidemic and pandemic risks. J Glob Health 12:05009 (2022). PubMed: 35265332
- Sriram G et al. The injury response to DNA damage in live tumor cells promotes antitumor immunity. Sci Signal 14:eabc4764 (2021). PubMed: 34665642
- Xu LZ et al. Chimeric specific antigen epitope-carrying dendritic cells induce interleukin-17(+) regulatory T cells to suppress food allergy. Clin Exp Allergy 50:231-243 (2020). PubMed: 31715648
- Barnett KC et al. Phosphoinositide Interactions Position cGAS at the Plasma Membrane to Ensure Efficient Distinction between Self- and Viral DNA. Cell 176:1432-1446.e11 (2019). PubMed: 30827685