The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use 1/50 - 1/200, or 10µl of the suggested working dilution to label 106 cells in 100µl.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceThree subregions of the Major Histocompatibility Complex (MHC), DP, DQ and DR, determine class II molecules, which are essential in the cellular recognition interactions involved in the generation of an immune response. These molecules display processed antigens from virally-infected or transformed cells. Class-II-positive cells (antigen-presenting cells) can take up antigens from outside by endocytosis, degrade them into small peptides, and re-export the peptides (now bound to MHC class II protein) to the cell surface. These peptide-MHC class II complexes can then be recognized by specific CD4+ lymphocytes. Several class II antigens are also associated with disease states.
The extremely high polymorphism of MHC class II transmembrane heterodimers is due to a few hypervariable segments present in the most external domain of their alpha and beta chains. The alpha chain of MHC-DR is not polymorphic, whereas the beta chain is highly so. Some changes in amino acid sequence are critical in disease susceptibility associations as well as the ability to present processed antigens to T cells.
Cellular localizationCell Membrane
DR alpha chain antibody
DR beta 5 antibody
HLA class II histocompatibility antigen antibody
HLA DRA antibody
HLA DRB1 antibody
MHC class II antigen DRA antibody
References for Anti-Ovine MHC Class II DR antibody [37.68] (ab34007)
has not yet been referenced specifically in any publications.
Publishing research using ab34007? Please let us know so that we can cite the reference in this datasheet.
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