Anti-Ovine MHC Class II DR antibody [37.68] (ab34007)

Overview

  • Product name
    Anti-Ovine MHC Class II DR antibody [37.68]
  • Description
    Mouse monoclonal [37.68] to Ovine MHC Class II DR
  • Tested applications
    Suitable for: Flow Cyt, IP, IHC-Frmore details
  • Species reactivity
    Reacts with: Sheep, Goat, Cow, Human
  • Immunogen

    Splenocytes from ATL mice

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Purified from tissue culture supernatant.
  • Clonality
    Monoclonal
  • Clone number
    37.68
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells.

Use 1/50 - 1/200, or 10µl of the suggested working dilution to label 106 cells in 100µl.

ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • Relevance
    Three subregions of the Major Histocompatibility Complex (MHC), DP, DQ and DR, determine class II molecules, which are essential in the cellular recognition interactions involved in the generation of an immune response. These molecules display processed antigens from virally-infected or transformed cells. Class-II-positive cells (antigen-presenting cells) can take up antigens from outside by endocytosis, degrade them into small peptides, and re-export the peptides (now bound to MHC class II protein) to the cell surface. These peptide-MHC class II complexes can then be recognized by specific CD4+ lymphocytes. Several class II antigens are also associated with disease states. The extremely high polymorphism of MHC class II transmembrane heterodimers is due to a few hypervariable segments present in the most external domain of their alpha and beta chains. The alpha chain of MHC-DR is not polymorphic, whereas the beta chain is highly so. Some changes in amino acid sequence are critical in disease susceptibility associations as well as the ability to present processed antigens to T cells.
  • Cellular localization
    Cell Membrane
  • Alternative names
    • DR alpha chain antibody
    • DR beta 5 antibody
    • HLA class II histocompatibility antigen antibody
    • HLA DRA antibody
    • HLA DRB1 antibody
    • MHC class II antigen DRA antibody
    see all

References

ab34007 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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