Anti-OVOL1 antibody [460C1a] (ab74520)
Key features and details
- Mouse monoclonal [460C1a] to OVOL1
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-OVOL1 antibody [460C1a]
See all OVOL1 primary antibodies -
Description
Mouse monoclonal [460C1a] to OVOL1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Recombinant fragment from the N-terminal of human OVOL1.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
ab74520 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and then filtered through a 0.22µm membrane. -
Clonality
Monoclonal -
Clone number
460C1a -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74520 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 30 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 30 kDa. |
Target
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Function
Putative transcription factor. Involved in hair formation and spermatogenesis. May function in the differentiation and/or maintenance of the urogenital system. -
Tissue specificity
Expressed in fetal kidney, and also in adult pancreas and placenta. Not expressed in intestine, peripheral blood lymphocytes and ovary. -
Sequence similarities
Contains 4 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- hOvo1 antibody
- Ovo homolog like 1 antibody
- Ovo like 1(Drosophila) antibody
see all
Images
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Anti-OVOL1 antibody [460C1a] (ab74520) + Immunising recombinant peptide
Predicted band size: 30 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
Datasheets and documents
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Datasheet download
References (2)
ab74520 has been referenced in 2 publications.
- Murata M et al. OVOL2-Mediated ZEB1 Downregulation May Prevent Promotion of Actinic Keratosis to Cutaneous Squamous Cell Carcinoma. J Clin Med 9:N/A (2020). PubMed: 32106476
- Tsuji G et al. Aryl hydrocarbon receptor activation restores filaggrin expression via OVOL1 in atopic dermatitis. Cell Death Dis 8:e2931 (2017). PubMed: 28703805