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Our Abpromise guarantee covers the use of ab83265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay, 1:62500.
Immunofluorescent staining of OVOL2 in human corneal endothelium with ab83265 at a dilution of 1/250. a: signal for Propidium iodide and OVOL2 b: signal for OVOL2 only c: corneal epithelium.
Corneal epithelium as well as limbal epithelium was positive in the cytoplasm. Stromal keratocytes and endothelium was positive predominantly in the cell nucleus
ab83265 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"