Overview

  • Product name
  • Description
    Rabbit polyclonal to OVOL2
  • Tested applications
    Suitable for: WB, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 226-275 (QEDLYLHVNS AHPGSSFLKK TSKKLAALLQ GKLTSAHQEN TSLSEEEERK) of human OVOL2 (NP_067043).

  • Positive control
    • 721_B cell lysate; human cornea

Properties

Applications

Our Abpromise guarantee covers the use of ab83265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay, 1:62500.

IHC-Fr 1/250.

Target

  • Function
    DNA-binding protein that binds to the 5'-G[GCT]GGGGG-3' core sequence. Probably acts as a transcription regulator.
  • Tissue specificity
    Expressed in testis, ovary, heart and skeletal muscle.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA504H3.3 antibody
    • EUROIMAGE566589 antibody
    • hOvo 2 antibody
    • hOvo2 antibody
    • Ovo like 2 (Drosophila) antibody
    • Ovo like 2 antibody
    • ovo-like zinc finger 2 antibody
    • OVOL 2 antibody
    • OVOL2 antibody
    • OVOL2_HUMAN antibody
    • Transcription factor Ovo like 2 antibody
    • Transcription factor Ovo-like 2 antibody
    • Zinc finger protein 339 antibody
    • ZNF 339 antibody
    • ZNF339 antibody
    see all

Anti-OVOL2 antibody images

  • Anti-OVOL2 antibody (ab83265) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 30 kDa
    Observed band size : 30 kDa
    Gel concentration: 12%
  • Immunofluorescent staining of OVOL2 in human corneal endothelium with ab83265 at a dilution of 1/250. a: signal for Propidium iodide and OVOL2 b: signal for OVOL2 only c: corneal epithelium.

    Corneal epithelium as well as limbal epithelium was positive in the cytoplasm. Stromal keratocytes and endothelium was positive predominantly in the cell nucleus

References for Anti-OVOL2 antibody (ab83265)

ab83265 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (P21 cornea)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium citrate buffer
Permeabilization
Yes - 0.2% Tween 20
Specification
P21 cornea
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
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Submitted Oct 04 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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