Overview

  • Product name
  • Description
    Rabbit polyclonal to OVOL2
  • Tested applications
    Suitable for: WB, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 226-275 (QEDLYLHVNS AHPGSSFLKK TSKKLAALLQ GKLTSAHQEN TSLSEEEERK) of human OVOL2 (NP_067043).

  • Positive control
    • 721_B cell lysate; human cornea

Properties

Applications

Our Abpromise guarantee covers the use of ab83265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay, 1:62500.

IHC-Fr 1/250.

Target

  • Function
    DNA-binding protein that binds to the 5'-G[GCT]GGGGG-3' core sequence. Probably acts as a transcription regulator.
  • Tissue specificity
    Expressed in testis, ovary, heart and skeletal muscle.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA504H3.3 antibody
    • EUROIMAGE566589 antibody
    • hOvo 2 antibody
    • hOvo2 antibody
    • Ovo like 2 (Drosophila) antibody
    • Ovo like 2 antibody
    • ovo-like zinc finger 2 antibody
    • OVOL 2 antibody
    • OVOL2 antibody
    • OVOL2_HUMAN antibody
    • Transcription factor Ovo like 2 antibody
    • Transcription factor Ovo-like 2 antibody
    • Zinc finger protein 339 antibody
    • ZNF 339 antibody
    • ZNF339 antibody
    see all

Images

  • Anti-OVOL2 antibody (ab83265) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 30 kDa
    Observed band size : 30 kDa
    Gel concentration: 12%
  • Immunofluorescent staining of OVOL2 in human corneal endothelium with ab83265 at a dilution of 1/250. a: signal for Propidium iodide and OVOL2 b: signal for OVOL2 only c: corneal epithelium.

    Corneal epithelium as well as limbal epithelium was positive in the cytoplasm. Stromal keratocytes and endothelium was positive predominantly in the cell nucleus

References

ab83265 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (P21 cornea)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium citrate buffer
Permeabilization
Yes - 0.2% Tween 20
Specification
P21 cornea
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
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Submitted Oct 04 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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