Overview

  • Product nameOxaloacetate Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Sensitivity
    > 2 µM
  • Range
    2 µM - 200 µM
  • Assay time
    0h 40m
  • Product overview

    Abcam's Oxaloacetate Assay Kit provides a simple, sensitive and rapid means of quantifying OAA in a variety of samples. In the assay, OAA is converted to pyruvate which is utilized to convert a nearly colorless probe to an intensely colored (?max= 570nm) and fluorescent (Ex/Em = 535/587nm) product. The Oxaloacetate Assay Kit can detect 0.1-10nmol (2-200 µM) of OAA.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Oxaloacetate (OAA, HOOC-CO-CH2-COOH) is a TCA cycle intermediate. It precedes citrate which is formed by the transfer of an acetyl group to OAA. OAA is formed by the deamidation of aspartate or condensation of CO2 with pyruvate or PEP. Since mammals do not possess the enzymatic machinery to form TCA cycle intermediates from acetyl CoA, OAA is one of the anaplerotic entry points via pyruvate and pyruvate carboxykinase

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

  • RelevanceOxaloacetate (OAA, HOOC-CO-CH2-COOH) is a TCA cycle intermediate. It precedes citrate which is formed by the transfer of an acetyl group to OAA. OAA is formed by the deamidation of aspartate or condensation of CO2 with pyruvate or PEP. Since mammals do not possess the enzymatic machinery to form TCA cycle intermediates from acetyl CoA, OAA is one of the anaplerotic entry points via pyruvate and pyruvate carboxykinase.
  • Alternative names
    • OAA
    • Oxaloacetic acid

Associated products

Applications

Our Abpromise guarantee covers the use of ab83428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Oxaloacetate Assay Kit images

  • Oxaloacetate fluorimetrically measured in biofluids (after spin column) showing concentration (micromolar)

  • Standard curve (fluorimetric): mean of duplicates (+/-SD) with background readings subtracted

  • Oxaloacetate standard curve generated following the kit protocol.

Protocols

References for Oxaloacetate Assay Kit (ab83428)

This product has been referenced in:
  • Long LH & Halliwell B The effects of oxaloacetate on hydrogen peroxide generation from ascorbate and epigallocatechin gallate in cell culture media: potential for altering cell metabolism. Biochem Biophys Res Commun 417:446-50 (2012). Functional Studies . Read more (PubMed: 22166196) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"