Overview

  • Product nameOxytocin
  • Description
    Reproductive hormone
  • Biological descriptionInvolved in many aspects of mammalian reproduction as well as other physiological processes such as bond pairing and cardiovascular homeostasis.
  • Purity> 98%

Properties

    Applications

    Our Abpromise guarantee covers the use of ab120186 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Oxytocin images

    • ab87312 staining oxytocin receptor in MCF7 cells treated with oxytocin (ab120186), by ICC/IF. Increase of cytoplasmatic oxytocin receptor localization and correspondent decrease in nuclear oxytocin receptor correlates with increased concentration of oxytocin, as described in literature.
      The cells were incubated at 37°C for 15 minutes in media containing different concentrations of ab120186 (oxytocin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab87312 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 donkey anti-goat polyclonal antibody (ab96931) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    References for Oxytocin (ab120186)

    This product has been referenced in:

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    Product Wall

    All products in our range are potentially hazardous and are strictly for laboratory research and development use only. They should not be used for pharmaceutical, veterinary, household, agricultural, food, cosmetic or any other human use. They should o...

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    As we do not currently undertake our own research I can only offer advice regarding the literature. The main difference between the free acid form and the non-free acid is a difference to the terminal end of the peptide. The non-free acid has a -NH2 gr...

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    We have not specifically tested ab52147 on C2C12 cells. However, we do have customer feedback regarding the use of this antibody to detect the phosphorylated protein in treated myometrial smooth muscle cells. Read More

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"