Overview

  • Product nameOxytocin ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Low 20 39.9pg/ml 12.6%
    Medium 20 121.4pg/ml 10.2%
    High 20 363.7pg/ml 13.3%
  • Sample type
    Cell culture supernatant, Milk, Urine, Serum, Plasma
  • Assay typeCompetitive
  • Sensitivity
    15 pg/ml
  • Range
    15.6 pg/ml - 1000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Saliva 90 % - %
    Urine 111 % - %
    Serum 104 % - %

  • Assay time
    25h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
    Predicted to work with: a wide range of other species
  • Product overview

    Abcam’s Oxytocin in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Oxytocin in Tissue Culture media, Human Serum, Saliva, Cerebrospinal Fluid and Urine.

    A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Oxytocin antigen and a polyclonal rabbit antibody specific to Oxytocin. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Oxytocin captured in the plate.

  • Notes

    Oxytocin is a neurohypophysial peptide which is produced in the paraventricular nuclei of the hypothalamus and stored in the posterior pituitary. The molecule consists of nine amino acids linked with a [1-6] disulfide bond and a semi-flexible carboxyamidated tail. A hormone once thought to be limited to female smooth muscle reproductive physiology, more current findings have determined that oxytocin also functions as a neurotransmitter, may be involved in neuropsychiatric disorders, social/sexual behavior and is important in male reproductive physiology. Oxytocin and the related neurohypophysial peptide, Arg-Vasopressin, maintain renal water and sodium balance.  

    Highly conserved across species boundaries, oxytocin-like neurohypophysial peptides are substituted primarily at residues 4 and/or 8. In the Oxytocin-like peptide, mesotocin, a common peptide found in some fishes, reptiles, amphibians, marsupials and nonmammalian tetrapods, the leucine at residue 8 is substituted for isoleucine. Acting in classical endocrine fashion, Oxytocin elicits regulatory effects by binding specific cell surface receptors which in turn initiate a secondary intracellular response cascade via a phosphoinositide signaling pathway.

    Cross Reactivity

    Compound% Cross Reactivity
    Mesotocin7.0
    Oxytocin100
    Arg8-Vasotocin7.5
    Ser4,Ile8-Oxytocin<0.2
    TRH<0.2
    Somatostatin<0.2
    Met-Enkephalin<0.2
    VIP<0.2
    Lys8-Vasopressin<0.2
    Arg8-Vasopressin<0.2
    α−ANP<0.2
    Growth Hormone<0.2
    Tocinoic acid<0.2
    Melanostatin<0.2
  • Tested applicationsCompetitive ELISAmore details
  • PlatformMicroplate

Properties

  • Cellular localizationSecreted
  • Alternative names
    • Ocytocin
    • OT
    • OT NPI
    • OXT
    • Oxytocin neurophysin 1
    • Oxytocin neurophysin I preproprotein
    • Oxytocin prepro (neurophysin I)
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab133050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Oxytocin ELISA Kit images

  • Representative Standard Curve using ab133050.

Protocols

References for Oxytocin ELISA Kit (ab133050)

ab133050 has not yet been referenced specifically in any publications.

Product Wall

The only control we offer is the standard provided in the kit which is a synthetic form of oxytocin.
Regardless of lot, the standard is always provided at one consistent concentration so that they end user can follow exactly the steps in the insert...

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The detection antibody used in this kit was replaced in this new lot. The new antibody is highly specific to Oxytocin and has only minimal cross reactivity to Mesotocin. And unfortunately we do not have any other kits available for detection of Mesotoc...

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Yes you are correct in assuming that the mesotocin seems to have a higher affinity for binding to the antibody than oxytocin given the 130% cross reactivity. This of course is not an issue for species where mesotocin is not really present in meaningful...

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Gracias por contactarnos.

El kit ab133050 tienen un precio de 475€. Actualmente no está en stock en nuestro almacén, pero tardaría como mucho dos semanas en llegarles.

Este kit no lo hemos testado con muest...

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Thank you for contacting us.

I have been in touch with the lab regarding use of ab133050 on Bobwhite Quail tissues as you requested. We have not validated the oxytocin kit for brain samples but it may work assuming there are sufficient amo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"