Overview

  • Product name
    Oxytocin ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Low 20 39.9pg/ml 12.6%
    Medium 20 121.4pg/ml 10.2%
    High 20 363.7pg/ml 13.3%
    Inter-assay
    Sample n Mean SD CV%
    Low 17 47pg/ml 20.9%
    Medium 17 145.1pg/ml 16.5%
    High 17 397.2pg/ml 11.8%
  • Sample type
    Cell culture supernatant, Milk, Urine, Serum, Plasma
  • Assay type
    Competitive
  • Sensitivity
    15 pg/ml
  • Range
    15.6 pg/ml - 1000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Saliva 90 % - %
    Urine 111 % - %
    Serum 104 % - %

  • Assay time
    25h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
    Predicted to work with: a wide range of other species
  • Product overview

    Abcam’s Oxytocin in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Oxytocin in Tissue Culture media, Human Serum, Saliva, Cerebrospinal Fluid and Urine.

    A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Oxytocin antigen and a polyclonal rabbit antibody specific to Oxytocin. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Oxytocin captured in the plate.

  • Notes

    Oxytocin is a neurohypophysial peptide which is produced in the paraventricular nuclei of the hypothalamus and stored in the posterior pituitary. The molecule consists of nine amino acids linked with a [1-6] disulfide bond and a semi-flexible carboxyamidated tail. A hormone once thought to be limited to female smooth muscle reproductive physiology, more current findings have determined that oxytocin also functions as a neurotransmitter, may be involved in neuropsychiatric disorders, social/sexual behavior and is important in male reproductive physiology. Oxytocin and the related neurohypophysial peptide, Arg-Vasopressin, maintain renal water and sodium balance.  

    Highly conserved across species boundaries, oxytocin-like neurohypophysial peptides are substituted primarily at residues 4 and/or 8. In the Oxytocin-like peptide, mesotocin, a common peptide found in some fishes, reptiles, amphibians, marsupials and nonmammalian tetrapods, the leucine at residue 8 is substituted for isoleucine. Acting in classical endocrine fashion, Oxytocin elicits regulatory effects by binding specific cell surface receptors which in turn initiate a secondary intracellular response cascade via a phosphoinositide signaling pathway.

    Cross Reactivity

    Compound% Cross Reactivity
    Mesotocin7.0
    Oxytocin100
    Arg8-Vasotocin7.5
    Ser4,Ile8-Oxytocin<0.2
    TRH<0.2
    Somatostatin<0.2
    Met-Enkephalin<0.2
    VIP<0.2
    Lys8-Vasopressin<0.2
    Arg8-Vasopressin<0.2
    α−ANP<0.2
    Growth Hormone<0.2
    Tocinoic acid<0.2
    Melanostatin<0.2
  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform
    Microplate

Properties

  • Cellular localization
    Secreted
  • Alternative names
    • Ocytocin
    • OT
    • OT NPI
    • OXT
    • Oxytocin neurophysin 1
    • Oxytocin neurophysin I preproprotein
    • Oxytocin prepro (neurophysin I)
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab133050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Oxytocin ELISA Kit images

  • Representative Standard Curve using ab133050.

Protocols

References for Oxytocin ELISA Kit (ab133050)

ab133050 has not yet been referenced specifically in any publications.

Product Wall

Areview for ab133050

Good Average 3/5 (Ease of Use)
Abreviews
We used ab133050 in order to quantify the amount of oxytocin in frozen rat brain tissue samples approx. 1mm2.
Immediately before running the assay we homogenized the samples in the ice cold complete lysis buffer recommended by abcam. The samples were then centrifuged once and loaded directly to the assay plate.
We were pleased to find that oxytocin was detected within the range of the standard curve.
Had a minor issue with the washes, the amount of washing buffer recommended per well was excessive and we reduced it from 400 to 350 microliter. Otherwise the instructions are clear and the kit is user-friendly.
Username

Mrs. Milly Kritman

Verified customer

Submitted Aug 28 2017

Oxytocin elisa kit

Good Below average 2/5 (Ease of Use)
Abreviews
The elisa kit is very friendly and works fine, but we had problems with the sample extraction procedure.
The extraction protocol was not clear for researchers that it’s the first time they met this procedure. For example you wrote that the volume of the washes with 0.1% TFA is between 10-25 ml (?!). When we evaporated the 3ml elution solution we found out that its take almost two days (?!).
We looked for "protein researchers" and they guided us how to do it properly.
Another comment when we washed the plate with 400µl as mention in the elisa procedure (13.8) the wash buffer spilled out of the walls, so we washed the wells with 380 µl.
Apart of that, the kit is very good. The standard curve we received was ok (attached curve fit that was calculated with 4 parameters logistic). Nonetheless, our samples were from rat serum and in many samples we received signals lower than the lowest standard (lower than 15.6 pg/ml). We understand that the oxytocin concentration in these samples is very low.
We tried to measure 3 samples as serum without the extraction procedure and we received at these samples signals lower than the lowest standard while when we measured them after extraction we received a detectable signal. Therefore, we understand that the extraction step is crucial for the detection.
Username

Dr. Shlomit Mizrachi

Verified customer

Submitted Jun 28 2017

Oxytocin Kit - ab133050

Good Average 3/5 (Ease of Use)
Abreviews
I conducted the analyzes according to the protocol suggested in the manual using the dilution of the sample (mouse serum) at 1: 5.
My only criticism is due to the complexity of the analysis of results (obtaining curve and calculations)
Username

Abcam user community

Verified customer

Submitted Aug 23 2016

Thank you for your inquiry.


I have contacted the lab to get more information for you. I have now received the following answer that in summary states that we unfortunately do not know what factors lead to matrix effects but know from expe...

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Thank you for contacting us. The laboratory that developed the oxytocin and Arg8 vasopressin ELISA kits, ab133050 and ab133028, was not able to produce data showing the differences in samples collected with or without aprotinin. I did find the followin...

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The detection antibody used in this kit was replaced in this new lot. The new antibody is highly specific to Oxytocin and has only minimal cross reactivity to Mesotocin. And unfortunately we do not have any other kits available for detection of Mesotoc...

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Yes you are correct in assuming that the mesotocin seems to have a higher affinity for binding to the antibody than oxytocin given the 130% cross reactivity. This of course is not an issue for species where mesotocin is not really present in meaningful...

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Gracias por contactarnos.

El kit ab133050 tienen un precio de 475€. Actualmente no está en stock en nuestro almacén, pero tardaría como mucho dos semanas en llegarles.

Este kit no lo hemos testado con muest...

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Thank you for contacting us.

I have been in touch with the lab regarding use of ab133050 on Bobwhite Quail tissues as you requested. We have not validated the oxytocin kit for brain samples but it may work assuming there are sufficient amo...

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The only control we offer is the standard provided in the kit which is a synthetic form of oxytocin.
Regardless of lot, the standard is always provided at one consistent concentration so that they end user can follow exactly the steps in the insert...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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