Oxytocin ELISA Kit (ab133050)
- Product nameOxytocin ELISA Kit
- Detection methodColorimetric
Intra-assay Sample n Mean SD CV% Low 20 39.9pg/ml 12.6% Medium 20 121.4pg/ml 10.2% High 20 363.7pg/ml 13.3% Inter-assay Sample n Mean SD CV% Low 17 47pg/ml 20.9% Medium 17 145.1pg/ml 16.5% High 17 397.2pg/ml 11.8%
- Sample typeCell culture supernatant, Milk, Urine, Serum, Plasma
- Assay typeCompetitive
- Sensitivity15 pg/ml
- Range15.6 pg/ml - 1000 pg/ml
Sample specific recovery Sample type Average % Range Saliva 90 % - % Urine 111 % - % Serum 104 % - %
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
Predicted to work with: a wide range of other species
- Product overview
ab133050 is a competitive immunoassay for the quantitative determination of Oxytocin in samples. The kit uses a polyclonal antibody to Oxytocin to bind, in a competitive manner, the Oxytocin in the standard or sample or an alkaline phosphatase molecule which has Oxytocin covalently attached to it. After a simultaneous incubation at 4°C the excess reagents are washed away and substrate is added. After a short incubation time the enzyme reaction is stopped and the yellow color generated read on a microplate reader at 405nm. The intensity of the bound yellow color is inversely proportional to the concentration of Oxytocin in either standards or samples. The measured optical density is used to calculate the concentration of Oxytocin.
Oxytocin is a neurohypophysial peptide which is produced in the paraventricular nuclei of the hypothalamus and stored in the posterior pituitary. The molecule consists of nine amino acids linked with a [1-6] disulfide bond and a semi-flexible carboxyamidated tail. A hormone once thought to be limited to female smooth muscle reproductive physiology, more current findings have determined that oxytocin also functions as a neurotransmitter, may be involved in neuropsychiatric disorders, social/sexual behavior and is important in male reproductive physiology. Oxytocin and the related neurohypophysial peptide, Arg-Vasopressin, maintain renal water and sodium balance.
Highly conserved across species boundaries, oxytocin-like neurohypophysial peptides are substituted primarily at residues 4 and/or 8. In the Oxytocin-like peptide, mesotocin, a common peptide found in some fishes, reptiles, amphibians, marsupials and nonmammalian tetrapods, the leucine at residue 8 is substituted for isoleucine. Acting in classical endocrine fashion, Oxytocin elicits regulatory effects by binding specific cell surface receptors which in turn initiate a secondary intracellular response cascade via a phosphoinositide signaling pathway.
Compound % Cross Reactivity Mesotocin 7.0 Oxytocin 100 Arg8-Vasotocin 7.5 Ser4,Ile8-Oxytocin <0.2 TRH <0.2 Somatostatin <0.2 Met-Enkephalin <0.2 VIP <0.2 Lys8-Vasopressin <0.2 Arg8-Vasopressin <0.2 α−ANP <0.2 Growth Hormone <0.2 Tocinoic acid <0.2 Melanostatin <0.2
- Tested applicationsCompetitive ELISA more details
- Storage instructionsPlease refer to protocols.
Components 1 x 96 tests Assay Buffer 1 x 27ml Goat anti-Rabbit IgG Microtiter Plate 1 unit Oxytocin Antibody 1 x 5ml Oxytocin Conjugate 1 x 5ml Oxytocin standard 1 x 0.5ml Plate Sealer 1 unit pNpp Substrate 1 x 20ml Stop Solution 1 x 5ml Wash Buffer Concentrate 1 x 27ml
- Research Areas
- Cellular localizationSecreted
- OT NPI
- Oxytocin neurophysin 1
- Oxytocin neurophysin I preproprotein
- Oxytocin prepro (neurophysin I)
Our Abpromise guarantee covers the use of ab133050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Oxytocin ELISA Kit (ab133050)
ab133050 has not yet been referenced specifically in any publications.