Overview

  • Product nameAnti-P cadherin antibody [6A9]
    See all P cadherin primary antibodies
  • Description
    Mouse monoclonal [6A9] to P cadherin
  • Tested applicationsSuitable for: ICC/IF, WB, IP, Blockingmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein corresponding to P cadherin. From A431 Trypsinized membranes.

  • Positive control
    • WB: 293T and A431 cell lysate. Mouse brain tissue lysate. ICC/IF: A2058, BEAS-2B and HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab19350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB 1/100 - 1/1000. Detects a band of approximately 120 kDa.
IP Use at an assay dependent concentration.
Blocking Use at an assay dependent concentration.

Target

  • FunctionCadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
  • Tissue specificityExpressed in some normal epithelial tissues and in some carcinoma cell lines.
  • Involvement in diseaseDefects in CDH3 are the cause of hypotrichosis with juvenile macular dystrophy (HJMD) [MIM:601553]. HJMD is a rare autosomal recessive disorder characterized by early hair loss heralding severe degenerative changes of the retinal macula and culminating in blindness during the second to third decade of life.
    Defects in CDH3 are the cause of ectodermal dysplasia with ectrodactyly and macular dystrophy (EEM) [MIM:225280]; also known as EEM syndrome, Albrectsen-Svendsen syndrome or Ohdo-Hirayama-Terawaki syndrome. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EEM is an autosomal recessive condition characterized by features of ectodermal dysplasia such as sparse eyebrows and scalp hair, and selective tooth agenesis associated with macular dystrophy and ectrodactyly.
  • Sequence similaritiesContains 5 cadherin domains.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CADH3_HUMAN antibody
    • Cadherin 3 antibody
    • Cadherin 3 precursor antibody
    • Cadherin 3 type 1 antibody
    • Cadherin-3 antibody
    • Cadp antibody
    • Calcium dependent adhesion protein placental antibody
    • CDH 3 antibody
    • CDH3 antibody
    • CDH3 protein antibody
    • CDHP antibody
    • HJMD antibody
    • P cadherin (placental) antibody
    • P-cadherin antibody
    • PCAD antibody
    • Placental cadherin antibody
    see all

Anti-P cadherin antibody [6A9] images

  • All lanes : Anti-P cadherin antibody [6A9] (ab19350) at 1/500 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : Mouse brain tissue lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    HRP-conjugated anti-mouse IgG
  • Immunocytochemistry/Immunofluorescent analysis of A2058 cells labelling P Cadherin (green) with ab19350. Cells were grown on chamber slides and fixed with formaldehyde. Cells were incubated without (control, right) or with the primary antibody at a 1/20 dilution overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue).

  • Immunocytochemistry/Immunofluorescent analysis of BEAS-2B cells labelling P Cadherin (green) with ab19350. Cells were grown on chamber slides and fixed with formaldehyde. Cells were incubated without (control, right) or with the primary antibody at a 1/20 dilution overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue).

  • Immunocytochemistry/Immunofluorescent analysis of HeLa cells labelling P Cadherin (green) with ab19350. Cells were grown on chamber slides and fixed with formaldehyde. Cells were incubated without (control, right) or with the primary antibody at a 1/20 dilution overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue).

References for Anti-P cadherin antibody [6A9] (ab19350)

This product has been referenced in:
  • Gautrot JE  et al. Mimicking normal tissue architecture and perturbation in cancer with engineered micro-epidermis. Biomaterials 33:5221-9 (2012). ICC/IF ; Human . Read more (PubMed: 22541538) »
  • Lewis JE  et al. Cross-talk between adherens junctions and desmosomes depends on plakoglobin. J Cell Biol 136:919-34 (1997). WB ; Human . Read more (PubMed: 9049256) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"