Anti-P cadherin antibody [MM0508-9V11] (ab89900)

Overview

  • Product name
    Anti-P cadherin antibody [MM0508-9V11]
    See all P cadherin primary antibodies
  • Description
    Mouse monoclonal [MM0508-9V11] to P cadherin
  • Tested applications
    Suitable for: WB, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human recombinant P cadherin extracellular domain.

Properties

  • Form
    Lyophilised:Reconstitute the antibody with 200µl sterile PBS and the final concentration is 0.5mg/ml.
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    filter sterilised
  • Clonality
    Monoclonal
  • Clone number
    MM0508-9V11
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab89900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 91 kDa.
IHC-Fr 1/50 - 1/200.
Flow Cyt 1/50 - 1/200. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
  • Tissue specificity
    Expressed in some normal epithelial tissues and in some carcinoma cell lines.
  • Involvement in disease
    Defects in CDH3 are the cause of hypotrichosis with juvenile macular dystrophy (HJMD) [MIM:601553]. HJMD is a rare autosomal recessive disorder characterized by early hair loss heralding severe degenerative changes of the retinal macula and culminating in blindness during the second to third decade of life.
    Defects in CDH3 are the cause of ectodermal dysplasia with ectrodactyly and macular dystrophy (EEM) [MIM:225280]; also known as EEM syndrome, Albrectsen-Svendsen syndrome or Ohdo-Hirayama-Terawaki syndrome. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EEM is an autosomal recessive condition characterized by features of ectodermal dysplasia such as sparse eyebrows and scalp hair, and selective tooth agenesis associated with macular dystrophy and ectrodactyly.
  • Sequence similarities
    Contains 5 cadherin domains.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CADH3_HUMAN antibody
    • Cadherin 3 antibody
    • Cadherin 3 precursor antibody
    • Cadherin 3 type 1 antibody
    • Cadherin-3 antibody
    • Cadp antibody
    • Calcium dependent adhesion protein placental antibody
    • CDH 3 antibody
    • CDH3 antibody
    • CDH3 protein antibody
    • CDHP antibody
    • HJMD antibody
    • P cadherin (placental) antibody
    • P-cadherin antibody
    • PCAD antibody
    • Placental cadherin antibody
    see all

Images

  • Overlay histogram showing A431 cells stained with ab89900 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89900, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References

ab89900 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Flow Cytometry
Sample
Human Cell (Human embryonic stem cells)
Permeabilization
No
Gating Strategy
Isotype control
Specification
Human embryonic stem cells
Fixation
unfixed
Username

Abcam user community

Verified customer

Submitted Jun 08 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human embryonic stem cells)
Permeabilization
Yes - 0.1% Triton
Specification
Human embryonic stem cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 26 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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