Anti-P cadherin antibody [MM0508-9V11] (ab89900)

Overview

  • Product nameAnti-P cadherin antibody [MM0508-9V11]
    See all P cadherin primary antibodies
  • Description
    Mouse monoclonal [MM0508-9V11] to P cadherin
  • Tested applicationsSuitable for: WB, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human recombinant P cadherin extracellular domain.

Properties

  • FormLyophilised:Reconstitute the antibody with 200µl sterile PBS and the final concentration is 0.5mg/ml.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesfilter sterilised
  • ClonalityMonoclonal
  • Clone numberMM0508-9V11
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab89900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 91 kDa.
IHC-Fr 1/50 - 1/200.
Flow Cyt 1/50 - 1/200. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionCadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
  • Tissue specificityExpressed in some normal epithelial tissues and in some carcinoma cell lines.
  • Involvement in diseaseDefects in CDH3 are the cause of hypotrichosis with juvenile macular dystrophy (HJMD) [MIM:601553]. HJMD is a rare autosomal recessive disorder characterized by early hair loss heralding severe degenerative changes of the retinal macula and culminating in blindness during the second to third decade of life.
    Defects in CDH3 are the cause of ectodermal dysplasia with ectrodactyly and macular dystrophy (EEM) [MIM:225280]; also known as EEM syndrome, Albrectsen-Svendsen syndrome or Ohdo-Hirayama-Terawaki syndrome. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EEM is an autosomal recessive condition characterized by features of ectodermal dysplasia such as sparse eyebrows and scalp hair, and selective tooth agenesis associated with macular dystrophy and ectrodactyly.
  • Sequence similaritiesContains 5 cadherin domains.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CADH3_HUMAN antibody
    • Cadherin 3 antibody
    • Cadherin 3 precursor antibody
    • Cadherin 3 type 1 antibody
    • Cadherin-3 antibody
    • Cadp antibody
    • Calcium dependent adhesion protein placental antibody
    • CDH 3 antibody
    • CDH3 antibody
    • CDH3 protein antibody
    • CDHP antibody
    • HJMD antibody
    • P cadherin (placental) antibody
    • P-cadherin antibody
    • PCAD antibody
    • Placental cadherin antibody
    see all

Anti-P cadherin antibody [MM0508-9V11] images

  • Overlay histogram showing A431 cells stained with ab89900 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89900, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References for Anti-P cadherin antibody [MM0508-9V11] (ab89900)

ab89900 has not yet been referenced specifically in any publications.

Product Wall

Application Flow Cytometry
Sample Human Cell (Human embryonic stem cells)
Permeabilization No
Gating Strategy Isotype control
Specification Human embryonic stem cells
Fixation unfixed
Username

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Submitted Jun 08 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human embryonic stem cells)
Permeabilization Yes - 0.1% Triton
Specification Human embryonic stem cells
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative Paraformaldehyde
Username

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Verified customer

Submitted May 26 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"