Anti-P Glycoprotein antibody [265/F4] (ab3083)


  • Product nameAnti-P Glycoprotein antibody [265/F4]
    See all P Glycoprotein primary antibodies
  • Description
    Mouse monoclonal [265/F4] to P Glycoprotein
  • SpecificityWe have data to indicate that this antibody may not cross react with Human. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Hamster
  • Immunogen

    Colchicine resistant viable Chinese Hamster Ovary.

  • Positive control
    • Drug resistant Chinese Hamster Ovary (CHO) cells.



Our Abpromise guarantee covers the use of ab3083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. Fix with acetone.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 170 kDa (predicted molecular weight: 170 kDa). 2 hours at RT.


  • FunctionEnergy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
  • Tissue specificityExpressed in liver, kidney, small intestine and brain.
  • Involvement in diseaseGenetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13) [MIM:612244]. Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases.
  • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Alternative names
    • ABC20 antibody
    • ABCB1 antibody
    • ATP binding cassette, sub family B (MDR/TAP), member 1 antibody
    • ATP-binding cassette sub-family B member 1 antibody
    • CD243 antibody
    • CLCS antibody
    • Colchicin sensitivity antibody
    • Doxorubicin resistance antibody
    • GP170 antibody
    • MDR1 antibody
    • MDR1_HUMAN antibody
    • Multidrug resistance 1 antibody
    • Multidrug resistance protein 1 antibody
    • P glycoprotein 1 antibody
    • P gp antibody
    • P-glycoprotein 1 antibody
    • PGY1 antibody
    see all

Anti-P Glycoprotein antibody [265/F4] images

  • All lanes : Anti-P Glycoprotein antibody [265/F4] (ab3083) at 1/2000 dilution

    Lane 1 : Nuclear lysate prepared from human DU145 cells
    Lane 2 : Nuclear lysate prepared from human DU145 cells
    Lane 3 : Nuclear lysate prepared from human DU145 cells
    Lane 4 : Nuclear lysate prepared from human DU145 cells

    Lysates/proteins at 20 µg per lane.

    Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/10000 dilution
    developed using the ECL technique

    Predicted band size : 170 kDa

    Exposure time : 30 seconds

    Image courtesy of an anonymous Abreview.

    See Abreview

References for Anti-P Glycoprotein antibody [265/F4] (ab3083)

This product has been referenced in:
  • Xu L  et al. Enhanced activity of doxorubicin in drug resistant A549 tumor cells by encapsulation of P-glycoprotein inhibitor in PLGA-based nanovectors. Oncol Lett 7:387-392 (2014). WB ; Human . Read more (PubMed: 24396453) »
  • Lathan B  et al. Immunological detection of Chinese hamster ovary cells expressing a multidrug resistance phenotype. Cancer Res 45:5064-9 (1985). Read more (PubMed: 2862990) »

See all 2 Publications for this product

Product Wall

Application IHC - Wholemount
Sample Zebrafish Embryo (Tail region, vasculature)
Specification Tail region, vasculature

Dr. Ryan MacDonald

Verified customer

Submitted Mar 12 2014

In reviewing the information for human and mouse G-GP (NCBI Reference Sequence: NP_000918.2, NP_035206.2) it does seem that these have an expected size of ~140kDA before modification. If the sample is run in a reduced denatured form I would expect the ...

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Thank you for your inquiry.

This product has been raised againstColchicine resistant viable Chinese Hamster Ovary tissue.Unfortunately, we have not mapped the epitope location for this particular antibody.

We aim to provide as...

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Thank you for contacting us.

We have not yet tested this product in IHC-P and so I am unable to provide spefic information for this product. I will attach our IHC-P protocol for your review however this has not been optimized for this produc...

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Thank you for your enquiry and your interest in our products.

Here are a few antibodies against the following targets which have been tested and characterized for Flow cytometry. Please take a look at the on-line datasheets for further inform...

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Application Western blot
Sample Human Cell lysate - nuclear (DU145 cells)
Loading amount 20 µg
Specification DU145 cells
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Nov 17 2011

Thank you for contacting us. ab3364; anti P Glycoprotein is no longer available in our catalogue. However we have similar products available to buy. The catalogue numbers are ab3083; ab3366; ab10333; ab80594; ab80626. Please check the da...

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Application Immunocytochemistry/ Immunofluorescence
Sample Cow Cell (Liver cell line)
Specification Liver cell line
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% triton X-100
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Apr 16 2008

Thank you for getting back to us. Your enquiry has been passed onto me by my colleague in customer services. I can confirm that the epitope information detailed on our antibody datasheet is correct. The antibody has been shown to work in Mouse, Rat...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. P Glycoprotein antibody [265/F4] (ab3083) has been shown to reacts with Hamster P Glycoprotein and not against the human homolog of this prote...

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