Anti-P Glycoprotein antibody [265/F4] - BSA and Azide free (ab80626)

Overview

  • Product nameAnti-P Glycoprotein antibody [265/F4] - BSA and Azide freeSee all P Glycoprotein primary antibodies ...
  • Description
    Mouse monoclonal [265/F4] to P Glycoprotein - BSA and Azide free
  • Specificityab80626 shows no reaction with parent drug-sensitive CHO cells. We have data to indicate that this antibody may not cross react with Human. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsWB, IHC-Fr, Flow Cyt, ICC/IF more details
  • Species reactivity
    Reacts with: Chinese Hamster
  • Immunogen

    Colchicine-resistant viable Chinese Hamster Ovary (CHO) cells, followed by purified P Glycoprotein from CHO cells.

  • Positive control
    • Drug resistant Chinese Hamster Ovary (CHO) cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab80626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
IHC-Fr
Flow Cyt
ICC/IF
  • Application notesFlow Cyt: Use at an assay dependent dilution.
    ICC/IF: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution. Fix with Acetone.
    WB: Use at a concentration of 1 - 2 µg/ml for 2hrs at RT. Predicted molecular weight: 141 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionEnergy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
    • Tissue specificityExpressed in liver, kidney, small intestine and brain.
    • Involvement in diseaseGenetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13) [MIM:612244]. Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases.
    • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
      Contains 2 ABC transmembrane type-1 domains.
      Contains 2 ABC transporter domains.
    • Cellular localizationMembrane.
    • Target information above from: UniProt accession P08183 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names
      • ABC20 antibody
      • ABCB1 antibody
      • ATP binding cassette sub family B MDR/TAP member 1 antibody
      • ATP binding cassette, sub family B (MDR/TAP), member 1 antibody
      • ATP-binding cassette sub-family B member 1 antibody
      • CD243 antibody
      • CD243 antigen antibody
      • CLCS antibody
      • Colchicin sensitivity antibody
      • Doxorubicin resistance antibody
      • GP170 antibody
      • MDR 1 antibody
      • MDR1 antibody
      • MDR1_HUMAN antibody
      • Multidrug resistance 1 antibody
      • Multidrug resistance protein 1 antibody
      • P glycoprotein 1 antibody
      • P gp antibody
      • P-glycoprotein 1 antibody
      • PGY1 antibody
      see all

    References for Anti-P Glycoprotein antibody [265/F4] - BSA and Azide free (ab80626)

    This product has been referenced in:
    • Lathan B  et al. Immunological detection of Chinese hamster ovary cells expressing a multidrug resistance phenotype. Cancer Res 45:5064-9 (1985). WB, ICC/IF, Flow Cyt, IHC-Fr ; Chinese Hamster . Read more (PubMed: 2862990) »

    See 1 Publication for this product

    Product Wall

    Thank you for contacting us. ab3364; anti P Glycoprotein is no longer available in our catalogue. However we have similar products available to buy. The catalogue numbers are ab3083; ab3366; ab10333; ab80594; ab80626. Please check the da...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"