P Glycoprotein antibody [4E3] (ab10333)

Product Name 

P Glycoprotein antibody [4E3]

Product type 

Primary antibodies

Description 

Mouse monoclonal [4E3] to P Glycoprotein

Immunogen 

Tissue / cell preparation (Human squamous lung cancer cell line SW-1573/500).

Reacts with 
(species key)

Hu

Specificity 

P-glycoprotein - 170 Kda (External Epitope) The 4E3 monoclonal antibody recognizes an epitope of the mdr1 encoded P-glycoprotein located on the exterior surface of the plasma membrane. 4E3 does not cross-react with the mdr3 gene product.

Tested applications 
(see key)

Flow Cyt, ICC/IF

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Flow Cyt: 1/10.
ICC: 1/10.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Cell Lines: Drug-sensitive parental cell lines and their multidrug-resistant derivatives. Tissue: Liver (positive staining detected along luminal surfaces of bile canaliculi) or colon (positive staining localized to luminal surface of secretory epithelium).

Cellular localization 

Integral membrane protein

Research areas 

Cancer >> Drug resistance >> P glycoproteins
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Signal Transduction >> Metabolism >> Plasma Membrane >> Channels
Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases

Relevance 

P Glycoprotein, the product of the MDR1 gene, is expressed in distinct non-malignant cells, typically cells with secretory and excretory functions. It is assumed to function as an ATP-dependent drug efflux pump with broad substrate specificity. The highest expression of P Glycoprotein has been observed in kidney (proximal tubules), liver (bile canaliculi), adrenal gland and intestine, suggesting that the primary role of P Glycoprotein is in the normal secretion of physiological metabolites and ingested chemicals into bile, urine and the lumen of the intestinal tract. Elevated levels of P Glycoprotein have also been reported in multidrug-resistant cell lines and in colon, endometrial, ovarian, and breast tumors, as well as in sarcomas and leukemias / lymphomas.

P-glycoprotein, the product of the MDR1 gene, is expressed in distinct non-malignant cells, typically cells with secretory and excretory functions. It is assumed to function as an ATP-dependent drug efflux pump with broad substrate specificity. The highest expression of P-glycoprotein has been observed in kidney (proximal tubules), liver (bile canaliculi), adrenal gland and intestine, suggesting that the primary role of P-glycoprotein is in the normal secretion of physiological metabolites and ingested chemicals into bile, urine and the lumen of the intestinal tract. Elevated levels of P-glycoprotein have also been reported in multidrug-resistant cell lines and in colon, endometrial, ovarian, and breast tumors, as well as in sarcomas and leukemias/lymphomas.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

4E3

Isotype 

IgG2a

Purity 

Protein G purified

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Store at +4°C.

References for P Glycoprotein antibody [4E3] (ab10333)

  This product has been used in:

Fiskus W et al. Molecular and biologic characterization and drug sensitivity of pan-histone deacetylase inhibitor-resistant acute myeloid leukemia cells. Blood 112:2896-905 (2008). PubMed: 18660379

If you publish research using ab10333 please let us know so that we can cite the reference on this datasheet.

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