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Products:Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases
Overview
Product name
Anti-P Glycoprotein antibody [4E3]
See all P Glycoprotein products (9) ...
Description
Mouse monoclonal [4E3] to P Glycoprotein
Specificity
P-glycoprotein - 170 Kda (External Epitope) The 4E3 monoclonal antibody recognizes an epitope of the mdr1 encoded P-glycoprotein located on the exterior surface of the plasma membrane. 4E3 does not cross-react with the mdr3 gene product.
Tested applications
ICC, Flow Cyt, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Tissue / cell preparation (Human squamous lung cancer cell line SW-1573/500).
Positive control
Cell Lines: Drug-sensitive parental cell lines and their multidrug-resistant derivatives. Tissue: Liver (positive staining detected along luminal surfaces of bile canaliculi) or colon (positive staining localized to luminal surface of secretory epithelium).
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Primary antibody notes
P-glycoprotein, the product of the MDR1 gene, is expressed in distinct non-malignant cells, typically cells with secretory and excretory functions. It is assumed to function as an ATP-dependent drug efflux pump with broad substrate specificity. The highest expression of P-glycoprotein has been observed in kidney (proximal tubules), liver (bile canaliculi), adrenal gland and intestine, suggesting that the primary role of P-glycoprotein is in the normal secretion of physiological metabolites and ingested chemicals into bile, urine and the lumen of the intestinal tract. Elevated levels of P-glycoprotein have also been reported in multidrug-resistant cell lines and in colon, endometrial, ovarian, and breast tumors, as well as in sarcomas and leukemias/lymphomas.
Clonality
Monoclonal
Clone number
4E3
Isotype
IgG2a
Research areas
Cancer >> Drug resistance >> P glycoproteins
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Signal Transduction >> Metabolism >> Plasma Membrane >> Channels
Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10333 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC
Flow Cyt
Application notes
Flow Cyt: 1/10.
ICC: 1/10.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
Tissue specificity
Expressed in liver, kidney, small intestine and brain.
Involvement in disease
Genetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13) [MIM:612244]. Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases.
Sequence similarities
Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains.
Cellular localization
Membrane.
Target information above from: UniProt accessionP08183
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ABC20 antibody
- ABCB1 antibody
- ATP binding cassette sub family B MDR/TAP member 1 antibody
see all
Database links
- Entrez Gene: 5243 Human
- Omim: 171050 Human
- SwissProt: P08183 Human
- Unigene: 489033 Human
References for Anti-P Glycoprotein antibody [4E3] (ab10333)
This product has been referenced in:
- Fiskus Wet al. Molecular and biologic characterization and drug sensitivity of pan-histone deacetylase inhibitor-resistant acute myeloid leukemia cells. Blood 112:2896-905 (2008).Read more (PubMed: 18660379) »
See 1 publication for this product
Publishing research using ab10333? Please let us know so that we can cite the reference in this datasheet
![P Glycoprotein antibody [4E3] for ICC/IF in Human (10333)](/ps/Reviews/Images/ab8308_36661.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"