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Recombinant fragment within Human P Glycoprotein aa 950 to the C-terminus. The exact sequence is proprietary.
Database link: P08183
This product is a recombinant rabbit monoclonal antibody.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab170903 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/250 - 1/600. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 141 kDa.
For optimal detection Abcam recommends not boiling the sample after lysis.
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: P glycoprotein knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab170903 observed at 160 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab170903 was shown to recognize P glycoprotein when P glycoprotein knockout samples were used, along with additional cross-reactive bands. Wild-type and P glycoprotein knockout samples were subjected to SDS-PAGE. ab170903 and ab8245 (loading control to GAPDH) were diluted 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemical analysis of paraffin-embedded Human uterus tissue, labeling P Glycoprotein using unpurified ab170903 at a 1/250 dilution.
Immunohistochemical analysis of paraffin-embedded Human liver tissue, labeling P Glycoprotein using unpurified ab170903 at a 1/250 dilution.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue, labeling P Glycoprotein using unpurified ab170903 at a 1/250 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"