Multidrug-resistant Chinese hamster ovary cell line (CHrC5).
Our Abpromise guarantee covers the use of ab3366 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|IHC-P||1/40. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (EDTA, pH 8-9 for example). 20-60 minutes at room temperature.|
|IP||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 18630504
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Immunocytochemical analysis of rat spinal cord endothelial cells (RSCECs) labeling P Glycoprotein with ab3366 at 3.35ug/ml.