![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - p130 antibody [130P215] - BSA and Azide free (ab80628) image](#p107p130-Primary-antibodies-ab80628-1.jpg)
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Products:Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Overview
Product name
Anti-p130 antibody [130P215] - BSA and Azide free
See all p130 products (2) ...
Description
Mouse monoclonal [130P215] to p130 - BSA and Azide free
Specificity
ab80628 reacts only with the un(der) phosphorylated form of p130 protein.
Tested applications
WB, IP, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Rat, Human
Immunogen
Synthetic peptide corresponding to amino acids 878-913 of p130
Epitope
Amino acids 878-913.
Positive control
A427 cells; Tonsil tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
ab80628 is purified from ascites fluid by Protein G chromatography.
Clonality
Monoclonal
Clone number
130P215
Isotype
IgG1
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - p130 antibody [130P215] - BSA and Azide free (ab80628)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - p130 antibody [130P215] - BSA and Azide free (ab80628) image](/ps/datasheet/images/80/ab80628/p107p130-Primary-antibodies-ab80628-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab80628 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 2 µg/ml.Predicted molecular weight: 130 kDa.(Incubate for 2 hours at room temperature.)
IP: Use at 2 µg/mg of lysate.
IHC-P: 1/100
ICC/IF: Use at an assay dependent dilution.
Target
Relevance
p130 is a key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.
Cellular localization
Nuclear
Alternative names
- 130 kDa retinoblastoma associated protein antibody
- PPAR alpha interacting complex protein 128 antibody
- PRB2 antibody
see all
Database links
- Entrez Gene: 5934 Human
- Entrez Gene: 81758 Rat
- Omim: 180203 Human
- SwissProt: Q08999 Human
- SwissProt: O55081 Rat
Anti-p130 antibody [130P215] - BSA and Azide free images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - p130 antibody [130P215] - BSA and Azide free (ab80628)
Formalin fixed, paraffin embedded human colon cancer stained with anti p130 (a different form of ab80628, containing BSA and azide) at a 1/100 dilution, using peroxidase conjugate and AEC chromogen. Note nuclear staining of tumor cells.
References for Anti-p130 antibody [130P215] - BSA and Azide free (ab80628)
ab80628 has not yet been referenced specifically in any publications.
Publishing research using ab80628? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"