Anti-p107 (phospho T369) antibody (ab111348)

Overview

  • Product nameAnti-p107 (phospho T369) antibody
    See all p107 primary antibodies
  • Description
    Rabbit polyclonal to p107 (phospho T369)
  • Specificityab111348 detects endogenous levels of p107 only when phosphorylated at threonine 369.
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from Human p107 around the phosphorylation site of threonine 369 (P-S-TP-P-L).

  • Positive control
    • Human lymph node tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab111348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionKey regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation. Forms a complex with adenovirus E1A and with SV40 large T antigen. May bind and modulate functionally certain cellular proteins with which T and E1A compete for pocket binding. May act as a tumor suppressor.
  • Sequence similaritiesBelongs to the retinoblastoma protein (RB) family.
  • Post-translational
    modifications
    Exists in both phosphorylated and unphosphorylated forms, and T, but not E1A, binds only to the unphosphorylated form. Cell-cycle arrest properties are inactivated by phosphorylation on Thr-332, Ser-640, Ser-964 and Ser-975 by CDK4.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 107 kDa retinoblastoma-associated protein antibody
    • Cellular protein 107 antibody
    • Cellular protein p107 antibody
    • CP107 antibody
    • MGC40006 antibody
    • p107 antibody
    • PRB1 antibody
    • RBL1 antibody
    • RBL1_HUMAN antibody
    • Retinoblastoma like protein 1 antibody
    • retinoblastoma-like 1 (p107) antibody
    • Retinoblastoma-like protein 1 antibody
    see all

Anti-p107 (phospho T369) antibody images

  • ab111348 at 1/50 dilution staining p107 in paraffin-embedded Human Lymph node tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.

References for Anti-p107 (phospho T369) antibody (ab111348)

ab111348 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse Inner Ears)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 40 µg
Specification Mouse Inner Ears
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Shikha Tarang

Verified customer

Submitted Aug 17 2015

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (6% gel)
Sample Mouse Tissue lysate - whole (Lung)
Specification Lung
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jul 14 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"