Overview

  • Product nameAnti-p114RhoGEF antibodySee all p114RhoGEF primary antibodies ...
  • Description
    Goat polyclonal to p114RhoGEF
  • Tested applicationsWB, IP, ICC/IF more details
  • Species reactivity
    Reacts with: Dog, Human
  • Immunogen

    Synthetic peptide: SAKEDASKEDVIFF, corresponding to C terminal amino acids 1037-1050 of Human p114RhoGEF.

  • Positive control
    • Human muscle and HepG2 extracts. Caco-2, HCE and MDCK cells.
  • General notes


    Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription and cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). p114RhoGEF is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Primary antibody notes Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription and cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). p114RhoGEF is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab10152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use at an assay dependent concentration. Can be blocked with Human p114RhoGEF peptide (ab23189).
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Target information above from: UniProt accession Q6ZSZ5 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • 114 kDa Rho specific guanine nucleotide exchange factor antibody
    • 114 kDa Rho-specific guanine nucleotide exchange factor antibody
    • ARHGEF 18 antibody
    • Arhgef18 antibody
    • ARHGI_HUMAN antibody
    • KIAA0521 antibody
    • MGC15913 antibody
    • p114 Rho GEF antibody
    • p114-Rho-GEF antibody
    • p114RhoGEF antibody
    • Rho guanine nucleotide exchange factor 18 antibody
    • Rho specific guanine nucleotide exchange factor p114 antibody
    • Rho-specific guanine nucleotide exchange factor p114 antibody
    • Rho/Rac guanine nucleotide exchange factor (GEF) 18 antibody
    • Rho/Rac guanine nucleotide exchange factor 18 antibody
    • SA RhoGEF antibody
    • SA-RhoGEF antibody
    • Septin associated RhoGEF antibody
    • Septin-associated RhoGEF antibody
    see all

References for Anti-p114RhoGEF antibody (ab10152)

This product has been referenced in:
  • Tsuji T  et al. Involvement of p114-RhoGEF and Lfc in Wnt-3a- and dishevelled-induced RhoA activation and neurite retraction in N1E-115 mouse neuroblastoma cells. Mol Biol Cell 21:3590-600 (2010). Read more (PubMed: 20810787) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"