![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - p130 antibody [SPM301], prediluted (ab75694) image](#p130.bmp)
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Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Rb
Overview
Product name
Anti-p130 antibody [SPM301], prediluted
See all p130 products (4) ...
Description
Mouse monoclonal [SPM301] to p130, prediluted
Tested applications
IHC-Pmore details
Cross reactivity
Reacts with
Rat, Human
Predicted to work with
Horse, Cow, Dog, Chimpanzee
Immunogen
Synthetic peptide: QCPELMMDRHLDQLLMCAIYVMANIM, corresponding to amino acids 878-913 of Human p130
QCPELMMDRH LDQLLMCAIY VMANIM
Positive control
A427 cells, tonsil and colon cancer tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
ab75694 was purified from ascites fluid by Protein G.
Clonality
Monoclonal
Clone number
SPM301
Isotype
IgG1
Light chain type
kappa
Research areas
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Rb family
Cancer >> Cell cycle >> Cell cycle inhibitors >> Rb
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Epigenetics and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> Rb
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Rb
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - p130 antibody [SPM301], prediluted (ab75694)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - p130 antibody [SPM301], prediluted (ab75694) image](/ps/datasheet/Images/75/ab75694/p130.bmp)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab75694 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
IHC-P
Application notes
IHC-P: Ready to use. Perform heat mediated antigen retrieval before commencing with IHC staining protocol, by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
p130 is related both in structure and function to the retinoblastoma tumor suppressor protein and p107 (collectively known as pocket proteins) and is known to regulate the activity of E2F transcription factors. E2F transcription factors regulate the expression of a number of genes important in cell proliferation, particularly those involved in the progression through G1 and into the S phase of the cell cycle. Binding of p130 converts E2F transcription factors from transcriptional activators to transcriptional repressors.
Cellular localization
Nuclear
Alternative names
- 130 kDa retinoblastoma associated protein antibody
- PRB2 antibody
- Rb2 antibody
see all
Database links
- Entrez Gene: 5934 Human
- Entrez Gene: 81758 Rat
- Omim: 180203 Human
- SwissProt: Q08999 Human
- SwissProt: O55081 Rat
Anti-p130 antibody [SPM301], prediluted images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - p130 antibody [SPM301], prediluted (ab75694)
ab75694, prediluted, staining p130 in formalin fixed, paraffin embedded human colon cancer tissue sections by Immunohistochemistry.
References for Anti-p130 antibody [SPM301], prediluted (ab75694)
ab75694 has not yet been referenced specifically in any publications.
Publishing research using ab75694? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"