Overview

  • Product nameAnti-p130 antibody
  • Description
    Mouse monoclonal to p130
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Orangutan
  • Immunogen

    Recombinant fragment: VTPVSTATHS LSRLHTMLTG LRNAPSEKLE QILRTCSRDP TQAIANRLKE MFEIYSQHFQ PDEDFSNCAK EIASKHFRFA EMLYYKVLES VIEQEQKRLG , corresponding to amino acids 416-516 of Human p130 with proprietary tag (NP_005602)

  • Positive control
    • 293 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab89459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa).
ELISA Use at an assay dependent dilution.

Target

  • Relevancep130 is a key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • 130 kDa retinoblastoma associated protein antibody
    • IB4 antibody
    • PPAR alpha interacting complex protein 128 antibody
    • PRB2 antibody
    • PRIC128 antibody
    • RB2 antibody
    • RBL2 antibody
    • Retinoblastoma like 2 antibody
    • retinoblastoma like 2 (p130) antibody
    • Retinoblastoma like protein 2 antibody
    • RETINOBLASTOMA RELATED GENE RB2 antibody
    see all

Anti-p130 antibody images

  • Anti-p130 antibody (ab89459) at 1 µg/ml + 293 cell lysate at 50 µg

    Predicted band size : 128 kDa
    Observed band size : 128 kDa
  • Detection limit for recombinant tagged ab89459 is approximately 1ng/ml as a capture antibody

References for Anti-p130 antibody (ab89459)

ab89459 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"