The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000 - 1/10000. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa).
Application notesIs unsuitable for Flow Cyt,ICC or IHC-P.
FunctionKey regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.
Sequence similaritiesBelongs to the retinoblastoma protein (RB) family.
Developmental stageG0-restricted expression.
Post-translational modificationsDuring G0 and early G1 phase of the cell cycle, phosphorylated on Ser-639 and on 5 sites within the domain B. Phosphorylation on Ser-672 in G1 leads to its ubiquitin-dependent proteolysis.
Western blot - p130 (phospho S672) antibody [EP2126Y] (ab76255)
All lanes : Anti-p130 (phospho S672) antibody [EP2126Y] (ab76255) at 1/10000 dilution
Lane 1 : Jurkat cell lysates, treated with AP Lane 2 : Jurkat cell lysates, untreated
Lysates/proteins at 10 µg/ml per lane.
Secondary goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 128 kDa Observed band size : 128 kDa
References for Anti-p130 (phospho S672) antibody [EP2126Y] (ab76255)
This product has been referenced in:
Bohrer LR et al. Androgens suppress EZH2 expression via retinoblastoma (RB) and p130-dependent pathways: a potential mechanism of androgen-refractory progression of prostate cancer. Endocrinology151:5136-45 (2010).
Read more (PubMed: 20881251) »