Overview

  • Product nameAnti-p130 (phospho S672) antibody [EP2126Y]
    See all p130 primary antibodies
  • Description
    Rabbit monoclonal [EP2126Y] to p130 (phospho S672)
  • Tested applicationsSuitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues surrounding Serine 672 of human p130

  • Positive control
    • Jurkat cell lysates, treated or untreated with AP.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab76255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/10000. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa).
IP 1/20.
  • Application notesIs unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • FunctionKey regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.
    • Sequence similaritiesBelongs to the retinoblastoma protein (RB) family.
    • Developmental stageG0-restricted expression.
    • Post-translational
      modifications
      During G0 and early G1 phase of the cell cycle, phosphorylated on Ser-639 and on 5 sites within the domain B. Phosphorylation on Ser-672 in G1 leads to its ubiquitin-dependent proteolysis.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 130 kDa retinoblastoma associated protein antibody
      • 130 kDa retinoblastoma-associated protein antibody
      • IB4 antibody
      • p130 antibody
      • PPAR-alpha-interacting complex protein 128 antibody
      • PRB2 antibody
      • Rb2 antibody
      • Rbl2 antibody
      • RBL2_HUMAN antibody
      • RBR 2 antibody
      • RBR-2 antibody
      • RBR2 antibody
      • Retinoblastoma like 2 antibody
      • Retinoblastoma-like protein 2 antibody
      • Retinoblastoma-related protein 2 antibody
      • RIC128 antibody
      see all

    Anti-p130 (phospho S672) antibody [EP2126Y] images

    • All lanes : Anti-p130 (phospho S672) antibody [EP2126Y] (ab76255) at 1/10000 dilution

      Lane 1 : Jurkat cell lysates, treated with AP
      Lane 2 : Jurkat cell lysates, untreated

      Lysates/proteins at 10 µg/ml per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 128 kDa
      Observed band size : 128 kDa

    References for Anti-p130 (phospho S672) antibody [EP2126Y] (ab76255)

    This product has been referenced in:
    • Bohrer LR  et al. Androgens suppress EZH2 expression via retinoblastoma (RB) and p130-dependent pathways: a potential mechanism of androgen-refractory progression of prostate cancer. Endocrinology 151:5136-45 (2010). WB ; Human . Read more (PubMed: 20881251) »

    See 1 Publication for this product

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