Anti-p130 (phospho S672) antibody [EP2126Y] (ab76255)
Key features and details
- Rabbit monoclonal [EP2126Y] to p130 (phospho S672)
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-p130 (phospho S672) antibody [EP2126Y]
See all p130 primary antibodies -
Description
Rabbit monoclonal [EP2126Y] to p130 (phospho S672) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat whole cell lysate, treated or untreated with AP. IP: Jurkat was serum starved for 24h, then treated with 10% FBS for 20h whole cell lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2126Y -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000 - 1/10000. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa).
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IP |
1/20.
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Notes |
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WB
1/5000 - 1/10000. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa). |
IP
1/20. |
Target
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Function
Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor. -
Sequence similarities
Belongs to the retinoblastoma protein (RB) family. -
Developmental stage
G0-restricted expression. -
Post-translational
modificationsDuring G0 and early G1 phase of the cell cycle, phosphorylated on Ser-639 and on 5 sites within the domain B. Phosphorylation on Ser-672 in G1 leads to its ubiquitin-dependent proteolysis. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5934 Human
- Omim: 180203 Human
- SwissProt: Q08999 Human
- Unigene: 513609 Human
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Alternative names
- 130 kDa retinoblastoma associated protein antibody
- 130 kDa retinoblastoma-associated protein antibody
- IB4 antibody
see all
Images
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All lanes : Anti-p130 (phospho S672) antibody [EP2126Y] (ab76255) at 1/10000 dilution
Lane 1 : Jurkat cell lysate, treated with AP
Lane 2 : Jurkat cell lysate, untreated
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-rabbit (HRP) at 1/2000 dilution
Predicted band size: 128 kDa
Observed band size: 128 kDaControl: Rabbit monoclonal [EP2141Y] to p130 (ab76234)
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Purified ab76255 at 1/20 dilution (1µg) immunoprecipitating p130 in Jurkat was serum starved for 24h, then treated with 10% FBS for 20h whole cell lysate.
Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) was serum starved for 24h, then treated with 10% FBS for 20h whole cell lysate 10µg
Lane 2 (+): ab76255 + Jurkat was serum starved for 24h, then treated with 10% FBS for 20h whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab76255 in Jurkat was serum starved for 24h, then treated with 10% FBS for 20h whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 90-130 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab76255 has been referenced in 9 publications.
- Knudsen ES et al. CDK/cyclin dependencies define extreme cancer cell-cycle heterogeneity and collateral vulnerabilities. Cell Rep 38:110448 (2022). PubMed: 35235778
- Verdugo-Sivianes EM et al. Mutation of SPINOPHILIN (PPP1R9B) found in human tumors promotes the tumorigenic and stemness properties of cells. Theranostics 11:3452-3471 (2021). PubMed: 33537097
- Su M et al. Coronavirus Porcine Epidemic Diarrhea Virus Nucleocapsid Protein Interacts with p53 To Induce Cell Cycle Arrest in S-Phase and Promotes Viral Replication. J Virol 95:e0018721 (2021). PubMed: 34037422
- Enrico TP et al. Cyclin F drives proliferation through SCF-dependent degradation of the retinoblastoma-like tumor suppressor p130/RBL2. Elife 10:N/A (2021). PubMed: 34851822
- Schade AE et al. Cyclin D-CDK4 relieves cooperative repression of proliferation and cell cycle gene expression by DREAM and RB. Oncogene 38:4962-4976 (2019). PubMed: 30833638
- Patel HK et al. Elacestrant (RAD1901) exhibits anti-tumor activity in multiple ER+ breast cancer models resistant to CDK4/6 inhibitors. Breast Cancer Res 21:146 (2019). PubMed: 31852484
- Álvaro-Blanco J et al. MAZ induces MYB expression during the exit from quiescence via the E2F site in the MYB promoter. Nucleic Acids Res 45:9960-9975 (2017). PubMed: 28973440
- Iwahori S et al. Human cytomegalovirus-encoded viral cyclin-dependent kinase (v-CDK) UL97 phosphorylates and inactivates the retinoblastoma protein-related p107 and p130 proteins. J Biol Chem 292:6583-6599 (2017). PubMed: 28289097
- Bohrer LR et al. Androgens suppress EZH2 expression via retinoblastoma (RB) and p130-dependent pathways: a potential mechanism of androgen-refractory progression of prostate cancer. Endocrinology 151:5136-45 (2010). WB ; Human . PubMed: 20881251