Overview

  • Product nameAnti-p15 INK4b antibodySee all p15 INK4b primary antibodies ...
  • Description
    Rabbit polyclonal to p15 INK4b
  • SpecificityWe have data to indicate that this antibody may not cross react with Human. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsICC/IF, IHC-P more details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide: HRDIARYLHAATGD, corresponding to C terminal amino acids 117-130 of Mouse p15 INK4b

  • Positive control
    • 3T3-L1 cells This antibody gave a positive result in IHC in the following FFPE tissue: Rat large intestine.

Properties

Applications

Our Abpromise guarantee covers the use of ab74647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionInteracts strongly with CDK4 and CDK6. Potent inhibitor. Potential effector of TGF-beta induced cell cycle arrest.
  • Sequence similaritiesBelongs to the CDKN2 cyclin-dependent kinase inhibitor family.
    Contains 4 ANK repeats.
  • Target information above from: UniProt accession P42772 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • CDK inhibitory protein antibody
    • CDK4B Inhibitor antibody
    • CDKN2B antibody
    • CDN2B_HUMAN antibody
    • Cyclin dependent kinase 4 inhibitor B antibody
    • Cyclin Dependent Kinase Inhibitor 2B antibody
    • Cyclin dependent kinase inhibitor 2B p15 inhibits CDK4 antibody
    • Cyclin dependent kinases 4 and 6 binding protein antibody
    • Cyclin-dependent kinase 4 inhibitor B antibody
    • Cyclin-dependent kinases 4 and 6 binding protein antibody
    • INK4B antibody
    • MTS 2 antibody
    • MTS-2 antibody
    • MTS2 antibody
    • Multiple tumor suppressor 2 antibody
    • Multiple Tumor Supressor 2 antibody
    • OTTHUMP00000021154 antibody
    • OTTHUMP00000021155 antibody
    • p14 CDK inhibitor antibody
    • p14 INK4b antibody
    • p14-INK4b antibody
    • P15 antibody
    • p15 CDK inhibitor antibody
    • p15 inhibits CDK4 antibody
    • p15 INK4b antibody
    • P15(INK4B) antibody
    • p15-INK4b antibody
    • p15INK4B antibody
    • TP 15 antibody
    • TP15 antibody
    see all

Anti-p15 INK4b antibody images

  • IHC image of p15 INK4b staining in Rat large intestine formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab74647, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-p15 INK4b antibody (ab74647)

ab74647 has not yet been referenced specifically in any publications.

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